MFN1

Protein identityUniProt · HPA

UniProt accession

Q8IWA4

Gene

MFN1

Ensembl

ENSG00000171109

Chromosome

3

Canonical length

741 aa

Protein class

Plasma proteins, Predicted intracellular proteins, Predicted membrane proteins, Transporters

Subcellular location

Mitochondria

Quaternary structure

Homodimer

OverviewNCBI Gene

The protein encoded by this gene is a mediator of mitochondrial fusion. This protein and mitofusin 2 are homologs of the Drosophila protein fuzzy onion (Fzo). They are mitochondrial membrane proteins that interact with each other to facilitate mitochondrial targeting. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

741 residues, UniProt reviewed canonical sequence.

>Q8IWA4|MFN1
     1  MAEPVSPLKH FVLAKKAITA IFDQLLEFVT EGSHFVEATY KNPELDRIAT EDDLVEMQGY
    61  KDKLSIIGEV LSRRHMKVAF FGRTSSGKSS VINAMLWDKV LPSGIGHITN CFLSVEGTDG
   121  DKAYLMTEGS DEKKSVKTVN QLAHALHMDK DLKAGCLVRV FWPKAKCALL RDDLVLVDSP
   181  GTDVTTELDS WIDKFCLDAD VFVLVANSES TLMNTEKHFF HKVNERLSKP NIFILNNRWD
   241  ASASEPEYME DVRRQHMERC LHFLVEELKV VNALEAQNRI FFVSAKEVLS ARKQKAQGMP
   301  ESGVALAEGF HARLQEFQNF EQIFEECISQ SAVKTKFEQH TIRAKQILAT VKNIMDSVNL
   361  AAEDKRHYSV EEREDQIDRL DFIRNQMNLL TLDVKKKIKE VTEEVANKVS CAMTDEICRL
   421  SVLVDEFCSE FHPNPDVLKI YKSELNKHIE DGMGRNLADR CTDEVNALVL QTQQEIIENL
   481  KPLLPAGIQD KLHTLIPCKK FDLSYNLNYH KLCSDFQEDI VFRFSLGWSS LVHRFLGPRN
   541  AQRVLLGLSE PIFQLPRSLA STPTAPTTPA TPDNASQEEL MITLVTGLAS VTSRTSMGII
   601  IVGGVIWKTI GWKLLSVSLT MYGALYLYER LSWTTHAKER AFKQQFVNYA TEKLRMIVSS
   661  TSANCSHQVK QQIATTFARL CQQVDITQKQ LEEEIARLPK EIDQLEKIQN NSKLLRNKAV
   721  QLENELENFT KQFLPSSNEE S

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against MFN1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Other membrane

Secreted

No

Transmembrane segments

2

Mean surface accessibility (rSASA)

0.32

Highest tissue expression

22 nTPM

Expression across tissuesHPA

ReferencesPubMed · IEDB

Publications for MFN1 from three distinct lines of evidence, kept separate because they answer different questions: whether antibodies are directed at the protein, whether a B-cell epitope has been mapped on it, and whether a T-cell epitope has. Each is labelled with its source.

Sources: PubMed — antigen-level antibody evidence from a custom retrieval. Records matching a controlled set of autoantibody terms (the MeSH descriptors Autoantibodies and Autoantigens, with title and abstract term variants) were obtained through NCBI E-utilities, and their titles and abstracts parsed for constructions that direct an antibody at a named protein rather than for co-occurrence. Captured names were resolved against UniProt nomenclature and each antigen adjudicated individually against the source text. Bibliographic records from PubMed and MeSH, U.S. National Library of Medicine; citation metrics from NIH iCite (Hutchins et al., PLoS Biology 2016). Titles link to PubMed; abstracts are not reproduced here. The NLM does not endorse this analysis.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.8

gnomAD pLI

0

gnomAD missense Z

1.22

DepMap mean gene effect

-0.09

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 16% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• GTP metabolic process
• mitochondrial fusion
• mitochondrion localization
• positive regulation of mitochondrial membrane potential

Molecular functions

• GTP binding
• GTPase activity
• identical protein binding

Cellular components

• membrane
• mitochondrial outer membrane
• mitochondrion
• outer mitochondrial membrane protein complex

Protein domainsUniProt · Pfam · InterPro

• Fzo/mitofusin HR2 domain
• Mitofusin family
• P-loop containing nucleoside triphosphate hydrolase
• Dynamin-type guanine nucleotide-binding (G) domain
• Dynamin, N-terminal
• Dynamin family
• fzo-like conserved region

KeywordsUniProt

• Coiled coil
• Cytoplasm
• GTP-binding
• Hydrolase
• Membrane
• Mitochondrion
• Mitochondrion outer membrane
• Nucleotide-binding
• Transmembrane
• Transmembrane helix
• Ubl conjugation

InteractionsUniProt · HPA

Protein binding partners of MFN1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• THG1L
• VAT1
• MARCHF5
• MFN2