Seroatlas · Human Serome Atlas

MARCHF5

E3 ubiquitin-protein ligase MARCHF5

Also known as: FLJ20445, MARCH-V, MARCH5, MARH5_HUMAN, MITOL, RNF153

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q9NX47
Gene: MARCHF5
Ensembl: ENSG00000198060
Chromosome: 10
Canonical length: 278 aa
Protein class: Enzymes, Metabolic proteins, Predicted membrane proteins
Subcellular location: Mitochondria
Quaternary structure: Homodimer

OverviewNCBI Gene

MARCH5 is a ubiquitin ligase of the mitochondrial outer membrane that plays a role in the control of mitochondrial morphology by regulating mitofusin-2 (MFN2; MIM 608507) and DRP1 (DNM1L; MIM 603850) (Nakamura et al., 2006 [PubMed 16936636]).[supplied by OMIM, Mar 2008]

Canonical amino-acid sequenceUniProt

278 residues, UniProt reviewed canonical sequence.

>Q9NX47|MARCHF5
     1  MPDQALQQML DRSCWVCFAT DEDDRTAEWV RPCRCRGSTK WVHQACLQRW VDEKQRGNST
    61  ARVACPQCNA EYLIVFPKLG PVVYVLDLAD RLISKACPFA AAGIMVGSIY WTAVTYGAVT
   121  VMQVVGHKEG LDVMERADPL FLLIGLPTIP VMLILGKMIR WEDYVLRLWR KYSNKLQILN
   181  SIFPGIGCPV PRIPAEANPL ADHVSATRIL CGALVFPTIA TIVGKLMFSS VNSNLQRTIL
   241  GGIAFVAIKG AFKVYFKQQQ YLRQAHRKIL NYPEQEEA

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against MARCHF5 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Other membrane
Secreted: No
Transmembrane segments: 4
Mean surface accessibility (rSASA): 0.41
Highest tissue expression: 19 nTPM

Expression across tissuesHPA

Tissue

liver: 19 nTPM
esophagus: 18 nTPM
skeletal muscle: 17 nTPM
bone marrow: 16 nTPM
thyroid gland: 16 nTPM
tongue: 15 nTPM

Single-cell type

esophageal apical cells: 685 nCPM
early spermatids: 210 nCPM
neutrophils: 172 nCPM
ocular epithelial cells: 149 nCPM
esophageal suprabasal cells: 119 nCPM
basal keratinocytes: 111 nCPM

Immune cell

neutrophil: 12 nTPM
intermediate monocyte: 7.5 nTPM
eosinophil: 7 nTPM
myeloid DC: 6.9 nTPM
non-classical monocyte: 6.7 nTPM
classical monocyte: 6 nTPM

Brain region

choroid plexus: 14 nTPM
cerebral cortex: 14 nTPM
spinal cord: 12 nTPM
basal ganglia: 12 nTPM
hippocampal formation: 12 nTPM
white matter: 12 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.39
gnomAD pLI: 0.89
DepMap mean gene effect: -0.45
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of MARCHF5 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads MARCHF5 as an antibody target. Whether an autoantibody or antibody against MARCHF5 could matter depends on whether native MARCHF5 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

MARCHF5 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label MARCHF5 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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