Seroatlas · Human Serome Atlas

MAGED4

Melanoma-associated antigen D4

Also known as: KIAA1859, MAGD4_HUMAN, MAGE-E1, MAGE1, MAGED4B, MGC3210

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q96JG8
Gene: MAGED4
Ensembl: ENSG00000154545
Chromosome: X
Canonical length: 741 aa
Protein class: Predicted intracellular proteins, Predicted membrane proteins

OverviewNCBI Gene

Predicted to be involved in negative regulation of transcription by RNA polymerase II. Predicted to be active in nucleus. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

741 residues, UniProt reviewed canonical sequence.

>Q96JG8|MAGED4
     1  MAEGSFSVQS ESYSVEDMDE GSDEVGEEEM VEGNDYEEFG AFGGYGTLTS FDIHILRAFG
    61  SLGPGLRILS NEPWELENPV LAQTLVEALQ LDPETLANET AARAANVARA AASNRAARAA
   121  AAAARTAFSQ VVASHRVATP QVSGEDTQPT TYAAEAQGPT PEPPLASPQT SQMLVTSKMA
   181  APEAPATSAQ SQTGSPAQEA ATEGPSSACA FSQAPCAREV DANRPSTAFL GQNDVFDFTQ
   241  PAGVSGMAFP RPKRPAPAQE AATEGPSAAS GVPQTGPGRE VAATRPKTTK SGKALAKTRW
   301  VEPQNVVAAA AAKAKMATSI PEPEGAAAAT AQHSAEPWAR MGGKRTKKSK HLDDEYESSE
   361  EERETPAVPP TWRASQPSLT VRAQLAPRPP MAPRSQIPSR HVLCLPPRNV TLLQERANKL
   421  VKYLMIKDYK KIPIKRADML KDVIREYDEH FPEIIERATY TLEKKFGIHL KEIDKEEHLY
   481  ILVCTRDSSA RLLGKTKDTP RLSLLLVILG VIFMNGNRAS EAVLWEALRK MGLRPGVRHP
   541  FLGDLRKLIT DDFVKQKYLE YKKIPNSNPP EYEFLWGLRA RHETSKMRVL RFIAQNQNRD
   601  PREWKAHFLE AVDDAFKTMD VDMAEEHARA QMRAQMNIGD EALIGRWSWD DIQVELLTWD
   661  EDGDFGDAWA RIPFAFWARY HQYILNSNRA NRRATWRAGV SSGTNGGAST SVLDGPSTSS
   721  TIRTRNAARA GASFFSWIQH R

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against MAGED4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.53
Highest tissue expression: 17 nTPM

Expression across tissuesHPA

Tissue

cerebral cortex: 17 nTPM
ovary: 14 nTPM
choroid plexus: 7.3 nTPM
endometrium: 6.5 nTPM
smooth muscle: 5.7 nTPM
cervix: 5.1 nTPM

Single-cell type

other brain neurons: 0.4 nCPM
oligodendrocyte progenitor cells: 0.3 nCPM
pituitary stem cells: 0.3 nCPM
brain inhibitory neurons: 0.2 nCPM
ependymal cells: 0.2 nCPM
astrocytes: 0.1 nCPM

Immune cell

naive B-cell: 8.3 nTPM
T-reg: 5.3 nTPM
plasmacytoid DC: 3 nTPM
memory B-cell: 2.7 nTPM
memory CD4 T-cell: 1.9 nTPM
total PBMC: 0.9 nTPM

Brain region

hypothalamus: 131 nTPM
basal ganglia: 75 nTPM
medulla oblongata: 65 nTPM
spinal cord: 62 nTPM
cerebral cortex: 61 nTPM
pons: 60 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about MAGED4.

Disease | ImmuneIEDB

Conditions an epitope on MAGED4 was assayed in.

oral squamous cell carcinoma T cell
head and neck squamous cell carcinoma T cell
melanoma T cell

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.85
gnomAD pLI: 0.32
gnomAD missense Z: 0.84

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

negative regulation of transcription by RNA polymerase II

Cellular components

nucleus

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of MAGED4 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

TRIM27

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads MAGED4 as an antibody target. Whether an autoantibody or antibody against MAGED4 could matter depends on whether native MAGED4 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

MAGED4 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label MAGED4 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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