MAFK
Transcription factor MafK
Also known as: MAFK_HUMAN, NFE2U, P18
Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene
Protein identityUniProt · HPA
- UniProt accession
- O60675
- Gene
- MAFK
- Ensembl
- ENSG00000198517
- Chromosome
- 7
- Canonical length
- 156 aa
- Protein class
- Predicted intracellular proteins, Transcription factors
- Subcellular location
- Nucleoplasm
- Quaternary structure
- Homodimer
OverviewNCBI Gene
The developmentally regulated expression of the globin genes depends on upstream regulatory elements termed locus control regions (LCRs). LCRs are associated with powerful enhancer activity that is mediated by the transcription factor NFE2 (nuclear factor erythroid-2). NFE2 recognition sites are also present in the gene promoters of 2 heme biosynthetic enzymes, porphobilinogen deaminase (PBGD; MIM 609806) and ferrochelatase (FECH; MIM 612386). NFE2 DNA-binding activity consists of a heterodimer containing an 18-kD Maf protein (MafF, MafG (MIM 602020), or MafK) and p45 (MIM 601490). Both subunits are members of the activator protein-1 superfamily of basic leucine zipper (bZIP) proteins (see MIM 165160). Maf homodimers suppress transcription at NFE2 sites.[supplied by OMIM, Nov 2008]
Canonical amino-acid sequenceUniProt
156 residues, UniProt reviewed canonical sequence.
>O60675|MAFK
1 MTTNPKPNKA LKVKKEAGEN APVLSDDELV SMSVRELNQH LRGLTKEEVT RLKQRRRTLK
61 NRGYAASCRI KRVTQKEELE RQRVELQQEV EKLARENSSM RLELDALRSK YEALQTFART
121 VARGPVAPSK VATTSVITIV KSTELSSTSV PFSAASLocalizationUniProt · AlphaFold · HPA
Whether an antibody against MAFK can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.
- Antibody reachability
- Intracellular
- Secreted
- No
- Transmembrane segments
- 0
- Mean surface accessibility (rSASA)
- 0.55
- Highest tissue expression
- 94 nTPM
Expression across tissuesHPA
Tissue
- blood vessel: 94 nTPM
- heart muscle: 76 nTPM
- skeletal muscle: 74 nTPM
- colon: 59 nTPM
- lung: 57 nTPM
- prostate: 46 nTPM
Single-cell type
- endometrial glandular cells: 124 nCPM
- syncytiotrophoblasts: 113 nCPM
- urothelial cells: 79 nCPM
- endometrial luminal cells: 72 nCPM
- endometrial ciliated cells: 66 nCPM
- ocular epithelial cells: 66 nCPM
Immune cell
- plasmacytoid DC: 1.5 nTPM
- eosinophil: 1.2 nTPM
- memory CD8 T-cell: 0.7 nTPM
- naive CD4 T-cell: 0.7 nTPM
- T-reg: 0.7 nTPM
- gdT-cell: 0.6 nTPM
Brain region
- white matter: 68 nTPM
- choroid plexus: 59 nTPM
- amygdala: 57 nTPM
- thalamus: 55 nTPM
- medulla oblongata: 54 nTPM
- cerebral cortex: 52 nTPM
Genetic constraint and essentialitygnomAD · DepMap
Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.
- gnomAD LOEUF (loss-of-function intolerance)
- 0.73
- gnomAD pLI
- 0.71
- gnomAD missense Z
- 1.36
- DepMap mean gene effect
- -0.08
- DepMap dependency class
- selective
Cancer expressionTCGA
Across TCGA tumor cohorts, this protein is over-expressed in roughly 7% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).
OntologyGO
Biological processes
- negative regulation of transcription by RNA polymerase II
- regulation of transcription by RNA polymerase II
Molecular functions
- DNA-binding transcription factor activity
- DNA-binding transcription factor activity, RNA polymerase II-specific
- DNA-binding transcription repressor activity, RNA polymerase II-specific
- RNA polymerase II cis-regulatory region sequence-specific DNA binding
- sequence-specific DNA binding
- transcription cis-regulatory region binding
Cellular components
Protein domainsUniProt · Pfam · InterPro
KeywordsUniProt
InteractionsUniProt · HPA
Protein binding partners of MAFK in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.
Antibody and autoantibody relevanceSeroatlas analysis
Seroatlas reads MAFK as an antibody target. Whether an autoantibody or antibody against MAFK could matter depends on whether native MAFK is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.
MAFK is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.
Annotation status
The present source text does not explicitly label MAFK as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.
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