Seroatlas · Human Serome Atlas

LEFTY2

Left-right determination factor 2

Also known as: EBAF, LEFTA, LEFTYA, LFTY2_HUMAN, TGFB4

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
O00292
Gene
LEFTY2
Ensembl
ENSG00000143768
Chromosome
1
Canonical length
366 aa
Protein class
Disease related genes, Predicted secreted proteins
Secretome location
Secreted in other tissues

OverviewNCBI Gene

This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate the mature protein, which plays a role in left-right asymmetry determination of organ systems during development. The protein may also play a role in endometrial bleeding. Mutations in this gene have been associated with left-right axis malformations, particularly in the heart and lungs. Some types of infertility have been associated with dysregulated expression of this gene in the endometrium. This gene is closely linked to both a related family member and a related pseudogene. This gene encodes multiple isoforms that may undergo similar proteolytic processing. [provided by RefSeq, Aug 2016]

Canonical amino-acid sequenceUniProt

366 residues, UniProt reviewed canonical sequence.

>O00292|LEFTY2
     1  MWPLWLCWAL WVLPLAGPGA ALTEEQLLGS LLRQLQLSEV PVLDRADMEK LVIPAHVRAQ
    61  YVVLLRRSHG DRSRGKRFSQ SFREVAGRFL ASEASTHLLV FGMEQRLPPN SELVQAVLRL
   121  FQEPVPKAAL HRHGRLSPRS AQARVTVEWL RVRDDGSNRT SLIDSRLVSV HESGWKAFDV
   181  TEAVNFWQQL SRPRQPLLLQ VSVQREHLGP LASGAHKLVR FASQGAPAGL GEPQLELHTL
   241  DLRDYGAQGD CDPEAPMTEG TRCCRQEMYI DLQGMKWAKN WVLEPPGFLA YECVGTCQQP
   301  PEALAFNWPF LGPRQCIASE TASLPMIVSI KEGGRTRPQV VSLPNMRVQK CSCASDGALV
   361  PRRLQP

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against LEFTY2 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Secreted
Secreted
Yes
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.34
Highest tissue expression
160 nTPM

Expression across tissuesHPA

Tissue

  • endometrium: 160 nTPM
  • cervix: 89 nTPM
  • choroid plexus: 50 nTPM
  • ovary: 41 nTPM
  • smooth muscle: 35 nTPM
  • blood vessel: 20 nTPM

Single-cell type

  • endometrial stromal cells: 1.7 nCPM
  • leydig cells: 1.6 nCPM
  • erythrocytes: 1.5 nCPM
  • decidual stromal cells: 1.1 nCPM
  • pituicytes/fscs: 1.1 nCPM
  • late spermatids: 1 nCPM

Immune cell

  • basophil: 0.2 nTPM
  • naive CD4 T-cell: 0.1 nTPM
  • neutrophil: 0.1 nTPM
  • classical monocyte: 0 nTPM
  • eosinophil: 0 nTPM
  • gdT-cell: 0 nTPM

Brain region

  • choroid plexus: 34 nTPM
  • thalamus: 1.6 nTPM
  • hippocampal formation: 1.3 nTPM
  • hypothalamus: 0.6 nTPM
  • white matter: 0.4 nTPM
  • basal ganglia: 0.3 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about LEFTY2.

Disease | AllUniProt

Conditions LEFTY2 is implicated in, by any mechanism.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.95
gnomAD pLI
0
gnomAD missense Z
0.58
DepMap mean gene effect
0.07
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads LEFTY2 as an antibody target. Whether an autoantibody or antibody against LEFTY2 could matter depends on whether native LEFTY2 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

LEFTY2 is annotated as secreted, so native LEFTY2 circulates and is directly accessible to antibodies. Secreted and cell-surface proteins are the autoantibody targets most likely to act like drugs, blocking or depleting the native protein.

Annotation status

The present source text does not explicitly label LEFTY2 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/LEFTY2. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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