KIFAP3

Protein identityUniProt · HPA

UniProt accession

Q92845

Gene

KIFAP3

Ensembl

ENSG00000075945

Chromosome

1

Canonical length

792 aa

Protein class

Plasma proteins, Predicted intracellular proteins

Subcellular location

Nucleoplasm,Microtubules,Basal body

OverviewNCBI Gene

The small G protein GDP dissociation stimulator (smg GDS) is a regulator protein having two activities on a group of small G proteins including the Rho and Rap1 family members and Ki-Ras; one is to stimulate their GDP/GTP exchange reactions, and the other is to inhibit their interactions with membranes. The protein encoded by this gene contains 9 'Armadillo' repeats and interacts with the smg GDS protein through these repeats. This protein, which is highly concentrated around the endoplasmic reticulum, is phosphorylated by v-src, and this phosphorylation reduces the affinity of the protein for smg GDS. It is thought that this protein serves as a linker between human chromosome-associated polypeptide (HCAP) and KIF3A/B, a kinesin superfamily protein in the nucleus, and that it plays a role in the interaction of chromosomes with an ATPase motor protein. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2011]

Canonical amino-acid sequenceUniProt

792 residues, UniProt reviewed canonical sequence.

>Q92845|KIFAP3
     1  MQGEDARYLK RKVKGGNIDV HPSEKALIVH YEVEATILGE MGDPMLGERK ECQKIIRLKS
    61  LNANTDITSL ARKVVEECKL IHPSKLNEVE QLLYYLQNRR DSLSGKEKKE KSSKPKDPPP
   121  FEGMEIDEVA NINDMDEYIE LLYEDIPDKV RGSALILQLA RNPDNLEELL LNETALGALA
   181  RVLREDWKQS VELATNIIYI FFCFSSFSQF HGLITHYKIG ALCMNIIDHE LKRHELWQEE
   241  LSKKKKAVDE DPENQTLRKD YEKTFKKYQG LVVKQEQLLR VALYLLLNLA EDTRTELKMR
   301  NKNIVHMLVK ALDRDNFELL ILVVSFLKKL SIFMENKNDM VEMDIVEKLV KMIPCEHEDL
   361  LNITLRLLLN LSFDTGLRNK MVQVGLLPKL TALLGNDNYK QIAMCVLYHI SMDDRFKSMF
   421  AYTDCIPQLM KMLFECSDER IDLELISFCI NLAANKRNVQ LICEGNGLKM LMKRALKFKD
   481  PLLMKMIRNI SQHDGPTKNL FIDYVGDLAA QISNDEEEEF VIECLGTLAN LTIPDLDWEL
   541  VLKEYKLVPY LKDKLKPGAA EDDLVLEVVI MIGTVSMDDS CAALLAKSGI IPALIELLNA
   601  QQEDDEFVCQ IIYVFYQMVF HQATRDVIIK ETQAPAYLID LMHDKNNEIR KVCDNTLDII
   661  AEYDEEWAKK IQSEKFRWHN SQWLEMVESR QMDESEQYLY GDDRIEPYIH EGDILERPDL
   721  FYNSDGLIAS EGAISPDFFN DYHLQNGDVV GQHSFPGSLG MDGFGQPVGI LGRPATAYGF
   781  RPDEPYYYGY GS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against KIFAP3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.31

Highest tissue expression

60 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.54

gnomAD pLI

0

gnomAD missense Z

1.91

DepMap mean gene effect

0.01

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 10% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• anterograde dendritic transport of neurotransmitter receptor complex
• cardiac muscle cell apoptotic process
• cilium organization
• intracellular protein localization
• microtubule-based movement
• microtubule-based process
• negative regulation of cardiac muscle cell apoptotic process
• negative regulation of neuroblast proliferation
• negative regulation of thymocyte apoptotic process
• neuroblast proliferation
• plus-end-directed vesicle transport along microtubule
• protein-containing complex assembly
• signal transduction
• thymocyte apoptotic process
• positive regulation of calcium-dependent cell-cell adhesion

Molecular functions

• intraciliary transport particle B binding
• kinesin binding
• protein phosphatase binding

Cellular components

• axoneme
• centrosome
• ciliary basal body
• ciliary tip
• ciliary transition zone
• cilium
• condensed nuclear chromosome
• cytosol
• dendrite cytoplasm
• endoplasmic reticulum
• glutamatergic synapse
• Golgi apparatus
• kinesin II complex
• microtubule cytoskeleton
• periciliary membrane compartment
• photoreceptor connecting cilium
• photoreceptor inner segment
• photoreceptor outer segment
• postsynapse

Protein domainsUniProt · Pfam · InterPro

• Armadillo
• Armadillo-like helical
• Armadillo-type fold
• Kinesin-associated protein 3
• Kinesin-associated protein (KAP)

KeywordsUniProt

• Phosphoprotein
• Repeat

InteractionsUniProt · HPA

Protein binding partners of KIFAP3 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• SMC3
• KIF3A
• KIF3B
• KIF3C