ITGAL

Protein identityUniProt · HPA

UniProt accession

P20701

Gene

ITGAL

Ensembl

ENSG00000005844

Chromosome

16

Canonical length

1170 aa

Protein class

CD markers, FDA approved drug targets, Predicted intracellular proteins, Predicted membrane proteins, Transporters

OverviewNCBI Gene

ITGAL encodes the integrin alpha L chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form the integrin lymphocyte function-associated antigen-1 (LFA-1), which is expressed on all leukocytes. LFA-1 plays a central role in leukocyte intercellular adhesion through interactions with its ligands, ICAMs 1-3 (intercellular adhesion molecules 1 through 3), and also functions in lymphocyte costimulatory signaling. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

1170 residues, UniProt reviewed canonical sequence.

>P20701|ITGAL
     1  MKDSCITVMA MALLSGFFFF APASSYNLDV RGARSFSPPR AGRHFGYRVL QVGNGVIVGA
    61  PGEGNSTGSL YQCQSGTGHC LPVTLRGSNY TSKYLGMTLA TDPTDGSILA CDPGLSRTCD
   121  QNTYLSGLCY LFRQNLQGPM LQGRPGFQEC IKGNVDLVFL FDGSMSLQPD EFQKILDFMK
   181  DVMKKLSNTS YQFAAVQFST SYKTEFDFSD YVKRKDPDAL LKHVKHMLLL TNTFGAINYV
   241  ATEVFREELG ARPDATKVLI IITDGEATDS GNIDAAKDII RYIIGIGKHF QTKESQETLH
   301  KFASKPASEF VKILDTFEKL KDLFTELQKK IYVIEGTSKQ DLTSFNMELS SSGISADLSR
   361  GHAVVGAVGA KDWAGGFLDL KADLQDDTFI GNEPLTPEVR AGYLGYTVTW LPSRQKTSLL
   421  ASGAPRYQHM GRVLLFQEPQ GGGHWSQVQT IHGTQIGSYF GGELCGVDVD QDGETELLLI
   481  GAPLFYGEQR GGRVFIYQRR QLGFEEVSEL QGDPGYPLGR FGEAITALTD INGDGLVDVA
   541  VGAPLEEQGA VYIFNGRHGG LSPQPSQRIE GTQVLSGIQW FGRSIHGVKD LEGDGLADVA
   601  VGAESQMIVL SSRPVVDMVT LMSFSPAEIP VHEVECSYST SNKMKEGVNI TICFQIKSLI
   661  PQFQGRLVAN LTYTLQLDGH RTRRRGLFPG GRHELRRNIA VTTSMSCTDF SFHFPVCVQD
   721  LISPINVSLN FSLWEEEGTP RDQRAQGKDI PPILRPSLHS ETWEIPFEKN CGEDKKCEAN
   781  LRVSFSPARS RALRLTAFAS LSVELSLSNL EEDAYWVQLD LHFPPGLSFR KVEMLKPHSQ
   841  IPVSCEELPE ESRLLSRALS CNVSSPIFKA GHSVALQMMF NTLVNSSWGD SVELHANVTC
   901  NNEDSDLLED NSATTIIPIL YPINILIQDQ EDSTLYVSFT PKGPKIHQVK HMYQVRIQPS
   961  IHDHNIPTLE AVVGVPQPPS EGPITHQWSV QMEPPVPCHY EDLERLPDAA EPCLPGALFR
  1021  CPVVFRQEIL VQVIGTLELV GEIEASSMFS LCSSLSISFN SSKHFHLYGS NASLAQVVMK
  1081  VDVVYEKQML YLYVLSGIGG LLLLLLIFIV LYKVGFFKRN LKEKMEAGRG VPNGIPAEDS
  1141  EQLASGQEAG DPGCLKPLHE KDSESGGGKD

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ITGAL can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Cell surface

Secreted

No

Transmembrane segments

1

Mean surface accessibility (rSASA)

0.29

Highest tissue expression

59 nTPM

Expression across tissuesHPA

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about ITGAL.

Disease | ImmuneIEDB

Conditions an epitope on ITGAL was assayed in.

• Lyme disease T cell

ReferencesPubMed · IEDB

Publications for ITGAL from three distinct lines of evidence, kept separate because they answer different questions: whether antibodies are directed at the protein, whether a B-cell epitope has been mapped on it, and whether a T-cell epitope has. Each is labelled with its source.

Sources: PubMed — antigen-level antibody evidence from a custom retrieval. Records matching a controlled set of autoantibody terms (the MeSH descriptors Autoantibodies and Autoantigens, with title and abstract term variants) were obtained through NCBI E-utilities, and their titles and abstracts parsed for constructions that direct an antibody at a named protein rather than for co-occurrence. Captured names were resolved against UniProt nomenclature and each antigen adjudicated individually against the source text. IEDB — curated epitope assays from the Immune Epitope Database (Vita et al., Nucleic Acids Research 2019). Bibliographic records from PubMed and MeSH, U.S. National Library of Medicine; citation metrics from NIH iCite (Hutchins et al., PLoS Biology 2016). Titles link to PubMed; abstracts are not reproduced here. The NLM does not endorse this analysis.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.41

gnomAD pLI

0

gnomAD missense Z

2.18

DepMap mean gene effect

0

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• cell adhesion
• cell-cell adhesion
• cell-matrix adhesion
• heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules
• inflammatory response
• integrin-mediated signaling pathway
• leukocyte cell-cell adhesion
• memory T cell extravasation
• phagocytosis
• receptor clustering
• signal transduction
• T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell

Molecular functions

• cell adhesion molecule binding
• ICAM-3 receptor activity
• metal ion binding
• protein-containing complex binding
• signaling receptor activity

Cellular components

• cell surface
• extracellular exosome
• integrin alphaL-beta2 complex
• integrin complex
• membrane
• plasma membrane
• specific granule membrane

Protein domainsUniProt · Pfam · InterPro

• Integrin alpha chain
• von Willebrand factor, type A
• FG-GAP repeat
• Integrin alpha beta-propellor
• Integrin alpha, first immunoglubulin-like domain
• Integrin alpha chain, C-terminal cytoplasmic region, conserved site
• Integrin alpha, N-terminal
• Integrin domain superfamily
• von Willebrand factor A-like domain superfamily
• Integrin alpha, second immunoglobulin-like domain
• von Willebrand factor type A domain
• Integrin alpha cytoplasmic region
• FG-GAP repeat
• Integrin alpha Ig-like domain 1
• Integrin alpha Ig-like domain 2

KeywordsUniProt

• Calcium
• Cell adhesion
• Cell membrane
• Disulfide bond
• Glycoprotein
• Host-virus interaction
• Integrin
• Magnesium
• Membrane
• Metal-binding
• Phagocytosis
• Phosphoprotein
• Receptor
• Repeat
• Signal
• Transmembrane
• Transmembrane helix

InteractionsUniProt · HPA

Protein binding partners of ITGAL in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• CD226
• THBD
• F11R
• ICAM1
• PTPRC