Seroatlas · Human Serome Atlas

IMPACT

Protein IMPACT

Also known as: IMPCT_HUMAN, RWDD5

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q9P2X3
Gene: IMPACT
Ensembl: ENSG00000154059
Chromosome: 18
Canonical length: 320 aa
Protein class: Predicted intracellular proteins
Subcellular location: Nucleoplasm

OverviewNCBI Gene

Predicted to enable protein sequestering activity. Predicted to be involved in several processes, including GCN2-mediated signaling; cellular response to amino acid starvation; and regulation of gene expression. Predicted to act upstream of or within negative regulation of protein phosphorylation. Predicted to be active in cytoplasm. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

320 residues, UniProt reviewed canonical sequence.

>Q9P2X3|IMPACT
     1  MAEGDAGSDQ RQNEEIEAMA AIYGEEWCVI DDCAKIFCIR ISDDIDDPKW TLCLQVMLPN
    61  EYPGTAPPIY QLNAPWLKGQ ERADLSNSLE EIYIQNIGES ILYLWVEKIR DVLIQKSQMT
   121  EPGPDVKKKT EEEDVECEDD LILACQPESS LKALDFDISE TRTEVEVEEL PPIDHGIPIT
   181  DRRSTFQAHL APVVCPKQVK MVLSKLYENK KIASATHNIY AYRIYCEDKQ TFLQDCEDDG
   241  ETAAGGRLLH LMEILNVKNV MVVVSRWYGG ILLGPDRFKH INNCARNILV EKNYTNSPEE
   301  SSKALGKNKK VRKDKKRNEH

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against IMPACT can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.37
Highest tissue expression: 32 nTPM

Expression across tissuesHPA

Tissue

testis: 32 nTPM
thyroid gland: 30 nTPM
parathyroid gland: 26 nTPM
breast: 24 nTPM
salivary gland: 24 nTPM
kidney: 23 nTPM

Single-cell type

early primary spermatocytes: 96 nCPM
differentiating spermatogonia: 71 nCPM
undifferentiated spermatogonia: 66 nCPM
breast lactating cells: 62 nCPM
megakaryocytes: 56 nCPM
late primary spermatocytes: 54 nCPM

Immune cell

basophil: 66 nTPM
eosinophil: 19 nTPM
intermediate monocyte: 12 nTPM
myeloid DC: 12 nTPM
classical monocyte: 9.6 nTPM
non-classical monocyte: 9.1 nTPM

Brain region

hypothalamus: 41 nTPM
midbrain: 27 nTPM
medulla oblongata: 22 nTPM
white matter: 22 nTPM
spinal cord: 22 nTPM
pons: 22 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.12
gnomAD pLI: 0
gnomAD missense Z: 0.17
DepMap mean gene effect: 0.08
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

cellular response to amino acid starvation
GCN2-mediated signaling
intracellular signal transduction
negative regulation of transcription by RNA polymerase II
neuron projection extension
positive regulation of neuron differentiation
regulation of translational initiation
regulation of cytoplasmic translational initiation in response to stress

Molecular functions

Cellular components

cytoplasm

Protein domainsUniProt · Pfam · InterPro

RWD domain
Ubiquitin-conjugating enzyme/RWD-like
Ribosomal protein uS5 domain 2-type superfamily
RWD domain
Impact, N-terminal
Uncharacterised protein family UPF0029, Impact, conserved site
Impact family
Impact, N-terminal domain superfamily
Impact N-terminal domain

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of IMPACT in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads IMPACT as an antibody target. Whether an autoantibody or antibody against IMPACT could matter depends on whether native IMPACT is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

IMPACT is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label IMPACT as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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