HTR1A

Protein identityUniProt · HPA

UniProt accession

P08908

Gene

HTR1A

Ensembl

ENSG00000178394

Chromosome

5

Canonical length

422 aa

Protein class

Disease related genes, FDA approved drug targets, G-protein coupled receptors, Predicted intracellular proteins, Predicted membrane proteins, Transporters

OverviewNCBI Gene

This gene encodes a G protein-coupled receptor for 5-hydroxytryptamine (serotonin), and belongs to the 5-hydroxytryptamine receptor subfamily. Serotonin has been implicated in a number of physiologic processes and pathologic conditions. Inactivation of this gene in mice results in behavior consistent with an increased anxiety and stress response. Mutation in the promoter of this gene has been associated with menstrual cycle-dependent periodic fevers. [provided by RefSeq, Jun 2012]

Canonical amino-acid sequenceUniProt

422 residues, UniProt reviewed canonical sequence.

>P08908|HTR1A
     1  MDVLSPGQGN NTTSPPAPFE TGGNTTGISD VTVSYQVITS LLLGTLIFCA VLGNACVVAA
    61  IALERSLQNV ANYLIGSLAV TDLMVSVLVL PMAALYQVLN KWTLGQVTCD LFIALDVLCC
   121  TSSILHLCAI ALDRYWAITD PIDYVNKRTP RRAAALISLT WLIGFLISIP PMLGWRTPED
   181  RSDPDACTIS KDHGYTIYST FGAFYIPLLL MLVLYGRIFR AARFRIRKTV KKVEKTGADT
   241  RHGASPAPQP KKSVNGESGS RNWRLGVESK AGGALCANGA VRQGDDGAAL EVIEVHRVGN
   301  SKEHLPLPSE AGPTPCAPAS FERKNERNAE AKRKMALARE RKTVKTLGII MGTFILCWLP
   361  FFIVALVLPF CESSCHMPTL LGAIINWLGY SNSLLNPVIY AYFNKDFQNA FKKIIKCKFC
   421  RQ

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against HTR1A can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Cell surface

Secreted

No

Transmembrane segments

7

Mean surface accessibility (rSASA)

0.4

Highest tissue expression

6.1 nTPM

Expression across tissuesHPA

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about HTR1A.

Disease | AllUniProt

Conditions HTR1A is implicated in, by any mechanism.

• Periodic fever, menstrual cycle-dependent (PFMC) MIM:614674

ReferencesPubMed · IEDB

Publications for HTR1A from three distinct lines of evidence, kept separate because they answer different questions: whether antibodies are directed at the protein, whether a B-cell epitope has been mapped on it, and whether a T-cell epitope has. Each is labelled with its source.

Sources: PubMed — antigen-level antibody evidence from a custom retrieval. Records matching a controlled set of autoantibody terms (the MeSH descriptors Autoantibodies and Autoantigens, with title and abstract term variants) were obtained through NCBI E-utilities, and their titles and abstracts parsed for constructions that direct an antibody at a named protein rather than for co-occurrence. Captured names were resolved against UniProt nomenclature and each antigen adjudicated individually against the source text. Bibliographic records from PubMed and MeSH, U.S. National Library of Medicine; citation metrics from NIH iCite (Hutchins et al., PLoS Biology 2016). Titles link to PubMed; abstracts are not reproduced here. The NLM does not endorse this analysis.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.7

gnomAD pLI

0.2

gnomAD missense Z

0.64

DepMap mean gene effect

-0.06

DepMap dependency class

none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 2% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• adenylate cyclase-inhibiting serotonin receptor signaling pathway
• adult behavior
• behavioral fear response
• chemical synaptic transmission
• exploration behavior
• G protein-coupled receptor signaling pathway
• G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger
• gamma-aminobutyric acid signaling pathway
• positive regulation of cell population proliferation
• regulation of behavior
• regulation of dopamine metabolic process
• regulation of hormone secretion
• regulation of serotonin secretion
• regulation of vasoconstriction
• serotonin metabolic process
• serotonin receptor signaling pathway

Molecular functions

• G protein-coupled serotonin receptor activity
• Gi/o-coupled serotonin receptor activity
• neurotransmitter receptor activity
• receptor-receptor interaction
• serotonin binding
• serotonin receptor activity

Cellular components

• dendrite
• plasma membrane
• synapse

Protein domainsUniProt · Pfam · InterPro

• G protein-coupled receptor, rhodopsin-like
• 5-hydroxytryptamine receptor family
• GPCR, rhodopsin-like, 7TM
• 7 transmembrane receptor (rhodopsin family)
• 5-Hydroxytryptamine 1A receptor

KeywordsUniProt

• Behavior
• Cell membrane
• Cell projection
• Disulfide bond
• G-protein coupled receptor
• Glycoprotein
• Membrane
• Receptor
• Transducer
• Transmembrane
• Transmembrane helix

InteractionsUniProt · HPA

Protein binding partners of HTR1A in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• GALR1
• GPR39
• YIF1B
• FGFR1