HK2

Protein identityUniProt · HPA

UniProt accession

P52789

Gene

HK2

Ensembl

ENSG00000159399

Chromosome

2

Canonical length

917 aa

Protein class

Cancer-related genes, Enzymes, Metabolic proteins, Plasma proteins, Predicted intracellular proteins

Subcellular location

Vesicles,Centrosome,Mitochondria,Cytosol

OverviewNCBI Gene

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 2, the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells. [provided by RefSeq, Apr 2009]

Canonical amino-acid sequenceUniProt

917 residues, UniProt reviewed canonical sequence.

>P52789|HK2
     1  MIASHLLAYF FTELNHDQVQ KVDQYLYHMR LSDETLLEIS KRFRKEMEKG LGATTHPTAA
    61  VKMLPTFVRS TPDGTEHGEF LALDLGGTNF RVLWVKVTDN GLQKVEMENQ IYAIPEDIMR
   121  GSGTQLFDHI AECLANFMDK LQIKDKKLPL GFTFSFPCHQ TKLDESFLVS WTKGFKSSGV
   181  EGRDVVALIR KAIQRRGDFD IDIVAVVNDT VGTMMTCGYD DHNCEIGLIV GTGSNACYME
   241  EMRHIDMVEG DEGRMCINME WGAFGDDGSL NDIRTEFDQE IDMGSLNPGK QLFEKMISGM
   301  YMGELVRLIL VKMAKEELLF GGKLSPELLN TGRFETKDIS DIEGEKDGIR KAREVLMRLG
   361  LDPTQEDCVA THRICQIVST RSASLCAATL AAVLQRIKEN KGEERLRSTI GVDGSVYKKH
   421  PHFAKRLHKT VRRLVPGCDV RFLRSEDGSG KGAAMVTAVA YRLADQHRAR QKTLEHLQLS
   481  HDQLLEVKRR MKVEMERGLS KETHASAPVK MLPTYVCATP DGTEKGDFLA LDLGGTNFRV
   541  LLVRVRNGKW GGVEMHNKIY AIPQEVMHGT GDELFDHIVQ CIADFLEYMG MKGVSLPLGF
   601  TFSFPCQQNS LDESILLKWT KGFKASGCEG EDVVTLLKEA IHRREEFDLD VVAVVNDTVG
   661  TMMTCGFEDP HCEVGLIVGT GSNACYMEEM RNVELVEGEE GRMCVNMEWG AFGDNGCLDD
   721  FRTEFDVAVD ELSLNPGKQR FEKMISGMYL GEIVRNILID FTKRGLLFRG RISERLKTRG
   781  IFETKFLSQI ESDCLALLQV RAILQHLGLE STCDDSIIVK EVCTVVARRA AQLCGAGMAA
   841  VVDRIRENRG LDALKVTVGV DGTLYKLHPH FAKVMHETVK DLAPKCDVSF LQSEDGSGKG
   901  AALITAVACR IREAGQR

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against HK2 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Other membrane

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.21

Highest tissue expression

134 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.49

gnomAD pLI

0

gnomAD missense Z

1.16

DepMap mean gene effect

-0.27

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• apoptotic mitochondrial changes
• canonical glycolysis
• cellular response to leukemia inhibitory factor
• establishment of protein localization to mitochondrion
• fructose 6-phosphate metabolic process
• glucose 6-phosphate metabolic process
• glucose metabolic process
• glycolytic process
• intracellular glucose homeostasis
• lactation
• maintenance of protein location in mitochondrion
• negative regulation of mitochondrial membrane permeability
• negative regulation of reactive oxygen species metabolic process
• positive regulation of angiogenesis
• positive regulation of type 2 mitophagy
• regulation of D-glucose import
• response to hypoxia
• response to ischemia

Molecular functions

• ATP binding
• D-glucose binding
• fructokinase activity
• glucokinase activity
• hexokinase activity

Cellular components

• centrosome
• cytosol
• membrane
• mitochondrial outer membrane
• mitochondrion
• sarcoplasmic reticulum

Protein domainsUniProt · Pfam · InterPro

• Hexokinase
• Hexokinase, binding site
• Hexokinase, N-terminal
• Hexokinase, C-terminal
• ATPase, nucleotide binding domain
• Hexokinase
• Hexokinase

KeywordsUniProt

• Acetylation
• Allosteric enzyme
• ATP-binding
• Cytoplasm
• Glycolysis
• Kinase
• Membrane
• Mitochondrion
• Mitochondrion outer membrane
• Nucleotide-binding
• Repeat
• Transferase

InteractionsUniProt · HPA

Protein binding partners of HK2 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• TIGAR
• UBQLN1
• VDAC1