TIGAR

Protein identityUniProt · HPA

UniProt accession

Q9NQ88

Gene

TIGAR

Ensembl

ENSG00000078237

Chromosome

12

Canonical length

270 aa

Protein class

Enzymes, Metabolic proteins, Predicted intracellular proteins

Subcellular location

Cytosol

OverviewNCBI Gene

This gene is regulated as part of the p53 tumor suppressor pathway and encodes a protein with sequence similarity to the bisphosphate domain of the glycolytic enzyme that degrades fructose-2,6-bisphosphate. The protein functions by blocking glycolysis and directing the pathway into the pentose phosphate shunt. Expression of this protein also protects cells from DNA damaging reactive oxygen species and provides some protection from DNA damage-induced apoptosis. The 12p13.32 region that includes this gene is paralogous to the 11q13.3 region. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

270 residues, UniProt reviewed canonical sequence.

>Q9NQ88|TIGAR
     1  MARFALTVVR HGETRFNKEK IIQGQGVDEP LSETGFKQAA AAGIFLNNVK FTHAFSSDLM
    61  RTKQTMHGIL ERSKFCKDMT VKYDSRLRER KYGVVEGKAL SELRAMAKAA REECPVFTPP
   121  GGETLDQVKM RGIDFFEFLC QLILKEADQK EQFSQGSPSN CLETSLAEIF PLGKNHSSKV
   181  NSDSGIPGLA ASVLVVSHGA YMRSLFDYFL TDLKCSLPAT LSRSELMSVT PNTGMSLFII
   241  NFEEGREVKP TVQCICMNLQ DHLNGLTETR

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against TIGAR can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.27

Highest tissue expression

2.9 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.55

gnomAD pLI

0.73

DepMap mean gene effect

0.06

DepMap dependency class

none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• cardiac muscle cell apoptotic process
• cellular response to cobalt ion
• cellular response to hypoxia
• DNA damage response
• fructose 2,6-bisphosphate metabolic process
• glucose catabolic process to lactate via pyruvate
• glycolytic process
• intestinal epithelial cell development
• mitophagy
• negative regulation of glucose catabolic process to lactate via pyruvate
• negative regulation of glycolytic process
• negative regulation of mitophagy
• negative regulation of programmed cell death
• negative regulation of reactive oxygen species metabolic process
• positive regulation of cardiac muscle cell apoptotic process
• positive regulation of DNA repair
• positive regulation of pentose-phosphate shunt
• reactive oxygen species metabolic process
• response to gamma radiation
• response to ischemia
• response to xenobiotic stimulus
• positive regulation of hexokinase activity
• regulation of pentose-phosphate shunt
• regulation of response to DNA damage checkpoint signaling

Molecular functions

• 6-bisphosphate 2-phosphatase activity
• fructose-2

Cellular components

• cytoplasm
• cytosol
• mitochondrial outer membrane
• nucleus

Protein domainsUniProt · Pfam · InterPro

• Phosphoglycerate/bisphosphoglycerate mutase, active site
• Histidine phosphatase superfamily, clade-1
• Histidine phosphatase superfamily
• Histidine phosphatase superfamily (branch 1)
• Phosphoglycerate Mutase

KeywordsUniProt

• Acetylation
• Apoptosis
• Autophagy
• Cytoplasm
• Hydrolase
• Mitochondrion
• Nucleus

InteractionsUniProt · HPA

Protein binding partners of TIGAR in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• HK2