Seroatlas · Human Serome Atlas

HACD3

Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 3

Also known as: B-ind1, HACD3_HUMAN, HSPC121, PTPLAD1

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q9P035
Gene
HACD3
Ensembl
ENSG00000074696
Chromosome
15
Canonical length
362 aa
Protein class
Enzymes, Metabolic proteins, Predicted membrane proteins
Subcellular location
Endoplasmic reticulum

OverviewNCBI Gene

Enables enzyme binding activity and very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase activity. Involved in fatty acid biosynthetic process; positive regulation by virus of viral protein levels in host cell; and positive regulation of viral genome replication. Located in endoplasmic reticulum and nuclear membrane. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

362 residues, UniProt reviewed canonical sequence.

>Q9P035|HACD3
     1  MENQVLTPHV YWAQRHRELY LRVELSDVQN PAISITENVL HFKAQGHGAK GDNVYEFHLE
    61  FLDLVKPEPV YKLTQRQVNI TVQKKVSQWW ERLTKQEKRP LFLAPDFDRW LDESDAEMEL
   121  RAKEEERLNK LRLESEGSPE TLTNLRKGYL FMYNLVQFLG FSWIFVNLTV RFCILGKESF
   181  YDTFHTVADM MYFCQMLAVV ETINAAIGVT TSPVLPSLIQ LLGRNFILFI IFGTMEEMQN
   241  KAVVFFVFYL WSAIEIFRYS FYMLTCIDMD WKVLTWLRYT LWIPLYPLGC LAEAVSVIQS
   301  IPIFNETGRF SFTLPYPVKI KVRFSFFLQI YLIMIFLGLY INFRHLYKQR RRRYGQKKKK
   361  IH

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against HACD3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Other membrane
Secreted
No
Transmembrane segments
6
Mean surface accessibility (rSASA)
0.32
Highest tissue expression
111 nTPM

Expression across tissuesHPA

Tissue

  • liver: 111 nTPM
  • choroid plexus: 108 nTPM
  • adrenal gland: 79 nTPM
  • cerebral cortex: 78 nTPM
  • kidney: 74 nTPM
  • hypothalamus: 74 nTPM

Single-cell type

  • retinal pigment epithelial cells: 345 nCPM
  • urothelial cells: 262 nCPM
  • cytotrophoblasts: 262 nCPM
  • hepatocytes: 236 nCPM
  • foveolar cells: 235 nCPM
  • adrenal medulla cells: 234 nCPM

Immune cell

  • MAIT T-cell: 36 nTPM
  • naive CD4 T-cell: 33 nTPM
  • naive CD8 T-cell: 33 nTPM
  • gdT-cell: 32 nTPM
  • memory CD8 T-cell: 30 nTPM
  • memory CD4 T-cell: 29 nTPM

Brain region

  • pons: 160 nTPM
  • hypothalamus: 159 nTPM
  • midbrain: 143 nTPM
  • white matter: 131 nTPM
  • thalamus: 130 nTPM
  • choroid plexus: 129 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.59
gnomAD pLI
0.01
DepMap mean gene effect
-0.06
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of HACD3 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads HACD3 as an antibody target. Whether an autoantibody or antibody against HACD3 could matter depends on whether native HACD3 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

HACD3 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label HACD3 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/HACD3. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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