GTF2A1
Transcription initiation factor IIA subunit 1
Also known as: TF2AA_HUMAN, TFIIA
Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene
Protein identityUniProt · HPA
- UniProt accession
- P52655
- Gene
- GTF2A1
- Ensembl
- ENSG00000165417
- Chromosome
- 14
- Canonical length
- 376 aa
- Protein class
- Predicted intracellular proteins
- Subcellular location
- Nucleoplasm,Cytosol
OverviewNCBI Gene
Accurate transcription initiation on TATA-containing class II genes involves the ordered assembly of RNA polymerase II (POLR2A; MIM 180660) and several general initiation factors (summarized by DeJong and Roeder, 1993 [PubMed 8224848]). One of these factors is TFIIA, which when purified from HeLa extracts consists of 35-, 19-, and 12-kD subunits.[supplied by OMIM, Jul 2010]
Canonical amino-acid sequenceUniProt
376 residues, UniProt reviewed canonical sequence.
>P52655|GTF2A1
1 MANSANTNTV PKLYRSVIED VINDVRDIFL DDGVDEQVLM ELKTLWENKL MQSRAVDGFH
61 SEEQQLLLQV QQQHQPQQQQ HHHHHHHQQA QPQQTVPQQA QTQQVLIPAS QQATAPQVIV
121 PDSKLIQHMN ASNMSAAATA ATLALPAGVT PVQQILTNSG QLLQVVRAAN GAQYIFQPQQ
181 SVVLQQQVIP QMQPGGVQAP VIQQVLAPLP GGISPQTGVI IQPQQILFTG NKTQVIPTTV
241 AAPTPAQAQI TATGQQQPQA QPAQTQAPLV LQVDGTGDTS SEEDEDEEED YDDDEEEDKE
301 KDGAEDGQVE EEPLNSEDDV SDEEGQELFD TENVVVCQYD KIHRSKNKWK FHLKDGIMNL
361 NGRDYIFSKA IGDAEWLocalizationUniProt · AlphaFold · HPA
Whether an antibody against GTF2A1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.
- Antibody reachability
- Intracellular
- Secreted
- No
- Transmembrane segments
- 0
- Mean surface accessibility (rSASA)
- 0.6
- Highest tissue expression
- 22 nTPM
Expression across tissuesHPA
Tissue
- thyroid gland: 22 nTPM
- thymus: 20 nTPM
- testis: 18 nTPM
- lymph node: 15 nTPM
- cerebral cortex: 15 nTPM
- tongue: 15 nTPM
Single-cell type
- breast myoepithelial cells: 114 nCPM
- plasma cells: 109 nCPM
- neutrophils: 108 nCPM
- corticotrophs: 93 nCPM
- early spermatids: 89 nCPM
- monocyte progenitors: 79 nCPM
Immune cell
- basophil: 7.4 nTPM
- non-classical monocyte: 2.6 nTPM
- plasmacytoid DC: 1.9 nTPM
- eosinophil: 1.3 nTPM
- gdT-cell: 1.3 nTPM
- memory CD4 T-cell: 1.2 nTPM
Brain region
- cerebellum: 46 nTPM
- cerebral cortex: 45 nTPM
- white matter: 31 nTPM
- hypothalamus: 30 nTPM
- basal ganglia: 29 nTPM
- amygdala: 27 nTPM
Genetic constraint and essentialitygnomAD · DepMap
Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.
- gnomAD LOEUF (loss-of-function intolerance)
- 0.28
- gnomAD pLI
- 0.99
- gnomAD missense Z
- 2.13
- DepMap mean gene effect
- -0.79
- DepMap dependency class
- common
Cancer expressionTCGA
Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).
OntologyGO
Biological processes
- positive regulation of transcription by RNA polymerase II
- positive regulation of transcription initiation by RNA polymerase II
- RNA polymerase II preinitiation complex assembly
- transcription by RNA polymerase II
Molecular functions
- DNA binding
- protein heterodimerization activity
- RNA polymerase II core promoter sequence-specific DNA binding
- RNA polymerase II general transcription initiation factor activity
- RNA polymerase II general transcription initiation factor binding
- RNA polymerase II-specific DNA-binding transcription factor binding
- TBP-class protein binding
Cellular components
Protein domainsUniProt · Pfam · InterPro
KeywordsUniProt
InteractionsUniProt · HPA
Protein binding partners of GTF2A1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.
Antibody and autoantibody relevanceSeroatlas analysis
Seroatlas reads GTF2A1 as an antibody target. Whether an autoantibody or antibody against GTF2A1 could matter depends on whether native GTF2A1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.
GTF2A1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.
Annotation status
The present source text does not explicitly label GTF2A1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.
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