FOXA1

Protein identityUniProt · HPA

UniProt accession

P55317

Gene

FOXA1

Ensembl

ENSG00000129514

Chromosome

14

Canonical length

472 aa

Protein class

Cancer-related genes, Predicted intracellular proteins, Transcription factors

Subcellular location

Nucleoplasm,Nucleoli fibrillar center

OverviewNCBI Gene

This gene encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

472 residues, UniProt reviewed canonical sequence.

>P55317|FOXA1
     1  MLGTVKMEGH ETSDWNSYYA DTQEAYSSVP VSNMNSGLGS MNSMNTYMTM NTMTTSGNMT
    61  PASFNMSYAN PGLGAGLSPG AVAGMPGGSA GAMNSMTAAG VTAMGTALSP SGMGAMGAQQ
   121  AASMNGLGPY AAAMNPCMSP MAYAPSNLGR SRAGGGGDAK TFKRSYPHAK PPYSYISLIT
   181  MAIQQAPSKM LTLSEIYQWI MDLFPYYRQN QQRWQNSIRH SLSFNDCFVK VARSPDKPGK
   241  GSYWTLHPDS GNMFENGCYL RRQKRFKCEK QPGAGGGGGS GSGGSGAKGG PESRKDPSGA
   301  SNPSADSPLH RGVHGKTGQL EGAPAPGPAA SPQTLDHSGA TATGGASELK TPASSTAPPI
   361  SSGPGALASV PASHPAHGLA PHESQLHLKG DPHYSFNHPF SINNLMSSSE QQHKLDFKAY
   421  EQALQYSPYG STLPASLPLG SASVTTRSPI EPSALEPAYY QGVYSRPVLN TS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against FOXA1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.64

Highest tissue expression

70 nTPM

Expression across tissuesHPA

ReferencesPubMed · IEDB

Publications for FOXA1 from three distinct lines of evidence, kept separate because they answer different questions: whether antibodies are directed at the protein, whether a B-cell epitope has been mapped on it, and whether a T-cell epitope has. Each is labelled with its source.

Sources: PubMed — antigen-level antibody evidence from a custom retrieval. Records matching a controlled set of autoantibody terms (the MeSH descriptors Autoantibodies and Autoantigens, with title and abstract term variants) were obtained through NCBI E-utilities, and their titles and abstracts parsed for constructions that direct an antibody at a named protein rather than for co-occurrence. Captured names were resolved against UniProt nomenclature and each antigen adjudicated individually against the source text. Bibliographic records from PubMed and MeSH, U.S. National Library of Medicine; citation metrics from NIH iCite (Hutchins et al., PLoS Biology 2016). Titles link to PubMed; abstracts are not reproduced here. The NLM does not endorse this analysis.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.68

gnomAD pLI

0.22

gnomAD missense Z

0.44

DepMap mean gene effect

-0.14

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• alveolar secondary septum development
• anatomical structure formation involved in morphogenesis
• anatomical structure morphogenesis
• cell differentiation
• chromatin remodeling
• dopaminergic neuron differentiation
• dorsal/ventral neural tube patterning
• epithelial cell maturation involved in prostate gland development
• epithelial tube branching involved in lung morphogenesis
• glucose homeostasis
• hormone metabolic process
• lung epithelial cell differentiation
• mesenchymal-epithelial cell signaling involved in prostate gland development
• negative regulation of epithelial to mesenchymal transition
• negative regulation of transcription by RNA polymerase II
• neuron fate specification
• Notch signaling pathway
• positive regulation of apoptotic process
• positive regulation of cell-cell adhesion mediated by cadherin
• positive regulation of DNA-binding transcription factor activity
• positive regulation of dopaminergic neuron differentiation
• positive regulation of intracellular estrogen receptor signaling pathway
• positive regulation of miRNA transcription
• positive regulation of mitotic cell cycle
• positive regulation of smoothened signaling pathway
• positive regulation of transcription by RNA polymerase II
• prostate gland epithelium morphogenesis
• prostate gland stromal morphogenesis
• regulation of transcription by RNA polymerase II
• respiratory basal cell differentiation
• response to estradiol
• secretory columnal luminar epithelial cell differentiation involved in prostate glandular acinus development
• smoothened signaling pathway

Molecular functions

• chromatin binding
• DNA binding
• DNA-binding transcription activator activity, RNA polymerase II-specific
• DNA-binding transcription factor activity
• DNA-binding transcription factor activity, RNA polymerase II-specific
• protein domain specific binding
• RNA polymerase II cis-regulatory region sequence-specific DNA binding
• sequence-specific double-stranded DNA binding
• transcription cis-regulatory region binding

Cellular components

• chromatin
• fibrillar center
• microvillus
• nucleoplasm
• nucleus

Protein domainsUniProt · Pfam · InterPro

• Fork head domain
• Fork-head N-terminal
• Fork head domain conserved site1
• Forkhead box protein, C-terminal
• Fork head domain conserved site 2
• Winged helix-like DNA-binding domain superfamily
• Winged helix DNA-binding domain superfamily
• Forkhead box domain-containing protein
• Forkhead domain
• Forkhead N-terminal region
• HNF3 C-terminal domain

KeywordsUniProt

• Activator
• Chromatin regulator
• Developmental protein
• DNA-binding
• Nucleus
• Phosphoprotein
• Repressor
• Transcription
• Transcription regulation

InteractionsUniProt · HPA

Protein binding partners of FOXA1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• AR
• FOXA2
• HDAC7
• NKX2-1
• NR0B2