Seroatlas · Human Serome Atlas

FLI1

Friend leukemia integration 1 transcription factor

Also known as: EWSR2, FLI-1, FLI1_HUMAN, SIC-1

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q01543
Gene
FLI1
Ensembl
ENSG00000151702
Chromosome
11
Canonical length
452 aa
Protein class
Cancer-related genes, Disease related genes, Human disease related genes, Predicted intracellular proteins, Transcription factors
Subcellular location
Nucleoplasm,Nuclear bodies
Quaternary structure
Homodimer

OverviewNCBI Gene

This gene encodes a transcription factor containing an ETS DNA-binding domain. The gene can undergo a t(11;22)(q24;q12) translocation with the Ewing sarcoma gene on chromosome 22, which results in a fusion gene that is present in the majority of Ewing sarcoma cases. An acute lymphoblastic leukemia-associated t(4;11)(q21;q23) translocation involving this gene has also been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]

Canonical amino-acid sequenceUniProt

452 residues, UniProt reviewed canonical sequence.

>Q01543|FLI1
     1  MDGTIKEALS VVSDDQSLFD SAYGAAAHLP KADMTASGSP DYGQPHKINP LPPQQEWINQ
    61  PVRVNVKREY DHMNGSRESP VDCSVSKCSK LVGGGESNPM NYNSYMDEKN GPPPPNMTTN
   121  ERRVIVPADP TLWTQEHVRQ WLEWAIKEYS LMEIDTSFFQ NMDGKELCKM NKEDFLRATT
   181  LYNTEVLLSH LSYLRESSLL AYNTTSHTDQ SSRLSVKEDP SYDSVRRGAW GNNMNSGLNK
   241  SPPLGGAQTI SKNTEQRPQP DPYQILGPTS SRLANPGSGQ IQLWQFLLEL LSDSANASCI
   301  TWEGTNGEFK MTDPDEVARR WGERKSKPNM NYDKLSRALR YYYDKNIMTK VHGKRYAYKF
   361  DFHGIAQALQ PHPTESSMYK YPSDISYMPS YHAHQQKVNF VPPHPSSMPV TSSSFFGAAS
   421  QYWTSPTGGI YPNPNVPRHP NTHVPSHLGS YY

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against FLI1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.55
Highest tissue expression
66 nTPM

Expression across tissuesHPA

Tissue

  • spleen: 66 nTPM
  • lymph node: 35 nTPM
  • tonsil: 35 nTPM
  • thymus: 31 nTPM
  • placenta: 31 nTPM
  • bone marrow: 25 nTPM

Single-cell type

  • neutrophils: 840 nCPM
  • neutrophil progenitors: 779 nCPM
  • megakaryocyte progenitors: 737 nCPM
  • vascular endothelial cells: 529 nCPM
  • microglia: 514 nCPM
  • monocyte progenitors: 404 nCPM

Immune cell

  • basophil: 54 nTPM
  • eosinophil: 37 nTPM
  • total PBMC: 29 nTPM
  • non-classical monocyte: 29 nTPM
  • myeloid DC: 26 nTPM
  • MAIT T-cell: 25 nTPM

Brain region

  • medulla oblongata: 17 nTPM
  • pons: 15 nTPM
  • thalamus: 14 nTPM
  • spinal cord: 13 nTPM
  • amygdala: 13 nTPM
  • cerebral cortex: 12 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about FLI1.

Disease | AllUniProt

Conditions FLI1 is implicated in, by any mechanism.

Disease | GeneticClinVar

9 pathogenic / likely-pathogenic of 302 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.28
gnomAD pLI
0.99
gnomAD missense Z
2.4
DepMap mean gene effect
-0.02
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of FLI1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads FLI1 as an antibody target. Whether an autoantibody or antibody against FLI1 could matter depends on whether native FLI1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

FLI1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label FLI1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/FLI1. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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