Seroatlas · Human Serome Atlas

FHL3

Four and a half LIM domains protein 3

Also known as: FHL3_HUMAN, SLIM2

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q13643
Gene
FHL3
Ensembl
ENSG00000183386
Chromosome
1
Canonical length
280 aa
Protein class
Predicted intracellular proteins
Subcellular location
Nucleoplasm

OverviewNCBI Gene

The protein encoded by this gene is a member of a family of proteins containing a four-and-a-half LIM domain, which is a highly conserved double zinc finger motif. The encoded protein has been shown to interact with the cancer developmental regulators SMAD2, SMAD3, and SMAD4, the skeletal muscle myogenesis protein MyoD, and the high-affinity IgE beta chain regulator MZF-1. This protein may be involved in tumor suppression, repression of MyoD expression, and repression of IgE receptor expression. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]

Canonical amino-acid sequenceUniProt

280 residues, UniProt reviewed canonical sequence.

>Q13643|FHL3
     1  MSESFDCAKC NESLYGRKYI QTDSGPYCVP CYDNTFANTC AECQQLIGHD SRELFYEDRH
    61  FHEGCFRCCR CQRSLADEPF TCQDSELLCN DCYCSAFSSQ CSACGETVMP GSRKLEYGGQ
   121  TWHEHCFLCS GCEQPLGSRS FVPDKGAHYC VPCYENKFAP RCARCSKTLT QGGVTYRDQP
   181  WHRECLVCTG CQTPLAGQQF TSRDEDPYCV ACFGELFAPK CSSCKRPIVG LGGGKYVSFE
   241  DRHWHHNCFS CARCSTSLVG QGFVPDGDQV LCQGCSQAGP

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against FHL3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.35
Highest tissue expression
1,502 nTPM

Expression across tissuesHPA

Tissue

  • skeletal muscle: 1,502 nTPM
  • tongue: 752 nTPM
  • colon: 42 nTPM
  • endometrium: 41 nTPM
  • esophagus: 35 nTPM
  • urinary bladder: 34 nTPM

Single-cell type

  • myonuclei: 379 nCPM
  • thymic myoid cells: 174 nCPM
  • smooth muscle cells: 88 nCPM
  • platelets: 62 nCPM
  • breast myoepithelial cells: 57 nCPM
  • decidual stromal cells: 51 nCPM

Immune cell

  • eosinophil: 337 nTPM
  • neutrophil: 35 nTPM
  • classical monocyte: 29 nTPM
  • intermediate monocyte: 18 nTPM
  • myeloid DC: 18 nTPM
  • MAIT T-cell: 15 nTPM

Brain region

  • spinal cord: 12 nTPM
  • pons: 11 nTPM
  • medulla oblongata: 11 nTPM
  • thalamus: 9.9 nTPM
  • white matter: 9.6 nTPM
  • midbrain: 8.8 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.85
gnomAD pLI
0
gnomAD missense Z
0.79
DepMap mean gene effect
0.02
DepMap dependency class
none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of FHL3 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads FHL3 as an antibody target. Whether an autoantibody or antibody against FHL3 could matter depends on whether native FHL3 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

FHL3 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label FHL3 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/FHL3. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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