FBXO4

Protein identityUniProt · HPA

UniProt accession

Q9UKT5

Gene

FBXO4

Ensembl

ENSG00000151876

Chromosome

5

Canonical length

387 aa

Protein class

Predicted intracellular proteins

Subcellular location

Nucleoplasm

Quaternary structure

Homodimer

OverviewNCBI Gene

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014]

Canonical amino-acid sequenceUniProt

387 residues, UniProt reviewed canonical sequence.

>Q9UKT5|FBXO4
     1  MAGSEPRSGT NSPPPPFSDW GRLEAAILSG WKTFWQSVSK ERVARTTSRE EVDEAASTLT
    61  RLPIDVQLYI LSFLSPHDLC QLGSTNHYWN ETVRDPILWR YFLLRDLPSW SSVDWKSLPD
   121  LEILKKPISE VTDGAFFDYM AVYRMCCPYT RRASKSSRPM YGAVTSFLHS LIIQNEPRFA
   181  MFGPGLEELN TSLVLSLMSS EELCPTAGLP QRQIDGIGSG VNFQLNNQHK FNILILYSTT
   241  RKERDRAREE HTSAVNKMFS RHNEGDDQQG SRYSVIPQIQ KVCEVVDGFI YVANAEAHKR
   301  HEWQDEFSHI MAMTDPAFGS SGRPLLVLSC ISQGDVKRMP CFYLAHELHL NLLNHPWLVQ
   361  DTEAETLTGF LNGIEWILEE VESKRAR

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against FBXO4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.32

Highest tissue expression

15 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.05

gnomAD pLI

0

gnomAD missense Z

0.65

DepMap mean gene effect

0.05

DepMap dependency class

none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 9% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• cellular homeostasis
• cellular response to ionizing radiation
• cellular senescence
• common myeloid progenitor cell proliferation
• negative regulation of fibroblast proliferation
• negative regulation of protein localization to nucleus
• positive regulation of protein polyubiquitination
• positive regulation of protein ubiquitination
• positive regulation of telomere maintenance via telomerase
• post-transcriptional regulation of gene expression
• protein destabilization
• protein polyubiquitination
• protein ubiquitination
• regulation of DNA damage checkpoint
• regulation of protein stability
• SCF-dependent proteasomal ubiquitin-dependent protein catabolic process
• telomere maintenance
• ubiquitin-dependent protein catabolic process

Molecular functions

• protein homodimerization activity
• ubiquitin protein ligase activity
• ubiquitin-like ligase-substrate adaptor activity
• ubiquitin-protein transferase activity

Cellular components

• cytoplasm
• cytosol
• SCF ubiquitin ligase complex
• ubiquitin ligase complex

Protein domainsUniProt · Pfam · InterPro

• F-box domain
• P-loop containing nucleoside triphosphate hydrolase
• F-box-like domain superfamily
• F-box-like
• F-box only protein 4

KeywordsUniProt

• Cytoplasm
• Phosphoprotein
• Ubl conjugation pathway

InteractionsUniProt · HPA

Protein binding partners of FBXO4 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• TERF1
• CUL1
• SKP1
• YWHAE