Seroatlas · Human Serome Atlas

FASTKD3

FAST kinase domain-containing protein 3, mitochondrial

Also known as: FAKD3_HUMAN, FLJ23274, MGC5297

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q14CZ7
Gene: FASTKD3
Ensembl: ENSG00000124279
Chromosome: 5
Canonical length: 662 aa
Protein class: Predicted intracellular proteins
Subcellular location: Nucleoplasm,Mitochondria

OverviewNCBI Gene

This gene encodes a member of a small family of Fas-activated serine/threonine kinase domain (FASTKD) containing proteins that share an amino terminal mitochondrial targeting domain and multiple carboxy terminal FAST domains as well as a putative RNA-binding RAP domain. The members of this family are ubiquitously expressed and are generally most abundant in mitochondria-enriched tissues such as heart, skeletal muscle and brown-adipose tissue. Some members of this protein family may play a role in apoptosis. The protein encoded by this gene interacts with components of the mitochondrial respiratory and translation networks. A pseudogene of this gene is also present on chromosome 5. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013]

Canonical amino-acid sequenceUniProt

662 residues, UniProt reviewed canonical sequence.

>Q14CZ7|FASTKD3
     1  MALITLRKNL YRLSDFQMHR ALAALKNKPL NHVHKVVKER LCPWLCSRQP EPFGVKFHHA
    61  HCKKFHSKNG NDLHPLGGPV FSQVSDCDRL EQNVKNEESQ MFYRRLSNLT SSEEVLSFIS
   121  TMETLPDTMA AGALQRICEV EKKDGDQGLP KEILENSIFQ ALCFQFEKEP SQLSNTSLVT
   181  ALQALILLHV DPQSSLLLNL VAECQNRLRK GGMEVRNLCI LGESLITLHS SGCVTLELII
   241  NQLQGEKLET FTPEDIVALY RILQACTEKV DEHQTFLNKI NNFSLSIVSN LSPKLISQML
   301  TALVVLDQSQ AFPLIIKLGK YVVRHVPHFT NEELRRVLEA FIYFGHHDTF FTKALEHRVA
   361  AVCLTLDPEV VCRVMEYCSR ELILSKPILN AVAETFVCQT EKFSPRQISA LMEPFGKLNY
   421  LPPNASALFR KLENVLFTHF NYFPPKSLLK LLHSCSLNEC HPVNFLAKIF KPLFLQRLQG
   481  KESHLDTLSR AQLTQLFLAS VLECPFYKGP KLLPKYQVKS FLTPCCSLET PVDSQLYRYV
   541  KIGLTNLLGA RLYFAPKVLT PYCYTIDVEI KLDEEGFVLP STANEDIHKR IALCIDGPKR
   601  FCSNSKHLLG KEAIKQRHLQ LLGYQVVQIP YHEIGMLKSR RELVEYLQRK LFSQNTVHWL
   661  QE

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against FASTKD3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.3
Highest tissue expression: 8.4 nTPM

Expression across tissuesHPA

Tissue

kidney: 8.4 nTPM
liver: 6.2 nTPM
rectum: 6.2 nTPM
tonsil: 6.1 nTPM
breast: 5.8 nTPM
small intestine: 5.6 nTPM

Single-cell type

paneth cells: 16 nCPM
gastric progenitor cells: 14 nCPM
decidual stromal cells: 13 nCPM
megakaryocytes: 12 nCPM
parietal cells: 12 nCPM
erythrocyte progenitors: 12 nCPM

Immune cell

T-reg: 8.2 nTPM
memory B-cell: 7.8 nTPM
naive CD4 T-cell: 7.2 nTPM
naive CD8 T-cell: 6.8 nTPM
memory CD4 T-cell: 6.7 nTPM
MAIT T-cell: 6.1 nTPM

Brain region

choroid plexus: 4.7 nTPM
white matter: 4.3 nTPM
cerebral cortex: 3.8 nTPM
basal ganglia: 3.5 nTPM
spinal cord: 3.4 nTPM
hypothalamus: 3.3 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.15
gnomAD pLI: 0
gnomAD missense Z: 0.81
DepMap mean gene effect: 0.06
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 13% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

RNA binding

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads FASTKD3 as an antibody target. Whether an autoantibody or antibody against FASTKD3 could matter depends on whether native FASTKD3 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

FASTKD3 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label FASTKD3 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Loading the interactive Seroatlas protein explorer...