FAP

Protein identityUniProt · HPA

UniProt accession

Q12884

Gene

FAP

Ensembl

ENSG00000078098

Chromosome

2

Canonical length

760 aa

Protein class

Cancer-related genes, Enzymes, Plasma proteins, Predicted intracellular proteins, Predicted secreted proteins

Secretome location

Secreted to blood

Quaternary structure

Homodimer

OverviewNCBI Gene

The protein encoded by this gene is a homodimeric integral membrane gelatinase belonging to the serine protease family. It is selectively expressed in reactive stromal fibroblasts of epithelial cancers, granulation tissue of healing wounds, and malignant cells of bone and soft tissue sarcomas. This protein is thought to be involved in the control of fibroblast growth or epithelial-mesenchymal interactions during development, tissue repair, and epithelial carcinogenesis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2014]

Canonical amino-acid sequenceUniProt

760 residues, UniProt reviewed canonical sequence.

>Q12884|FAP
     1  MKTWVKIVFG VATSAVLALL VMCIVLRPSR VHNSEENTMR ALTLKDILNG TFSYKTFFPN
    61  WISGQEYLHQ SADNNIVLYN IETGQSYTIL SNRTMKSVNA SNYGLSPDRQ FVYLESDYSK
   121  LWRYSYTATY YIYDLSNGEF VRGNELPRPI QYLCWSPVGS KLAYVYQNNI YLKQRPGDPP
   181  FQITFNGREN KIFNGIPDWV YEEEMLATKY ALWWSPNGKF LAYAEFNDTD IPVIAYSYYG
   241  DEQYPRTINI PYPKAGAKNP VVRIFIIDTT YPAYVGPQEV PVPAMIASSD YYFSWLTWVT
   301  DERVCLQWLK RVQNVSVLSI CDFREDWQTW DCPKTQEHIE ESRTGWAGGF FVSTPVFSYD
   361  AISYYKIFSD KDGYKHIHYI KDTVENAIQI TSGKWEAINI FRVTQDSLFY SSNEFEEYPG
   421  RRNIYRISIG SYPPSKKCVT CHLRKERCQY YTASFSDYAK YYALVCYGPG IPISTLHDGR
   481  TDQEIKILEE NKELENALKN IQLPKEEIKK LEVDEITLWY KMILPPQFDR SKKYPLLIQV
   541  YGGPCSQSVR SVFAVNWISY LASKEGMVIA LVDGRGTAFQ GDKLLYAVYR KLGVYEVEDQ
   601  ITAVRKFIEM GFIDEKRIAI WGWSYGGYVS SLALASGTGL FKCGIAVAPV SSWEYYASVY
   661  TERFMGLPTK DDNLEHYKNS TVMARAEYFR NVDYLLIHGT ADDNVHFQNS AQIAKALVNA
   721  QVDFQAMWYS DQNHGLSGLS TNHLYTHMTH FLKQCFSLSD

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against FAP can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Cell surface

Secreted

No

Transmembrane segments

1

Mean surface accessibility (rSASA)

0.23

Highest tissue expression

29 nTPM

Expression across tissuesHPA

ReferencesPubMed · IEDB

Publications for FAP from three distinct lines of evidence, kept separate because they answer different questions: whether antibodies are directed at the protein, whether a B-cell epitope has been mapped on it, and whether a T-cell epitope has. Each is labelled with its source.

Sources: PubMed — antigen-level antibody evidence from a custom retrieval. Records matching a controlled set of autoantibody terms (the MeSH descriptors Autoantibodies and Autoantigens, with title and abstract term variants) were obtained through NCBI E-utilities, and their titles and abstracts parsed for constructions that direct an antibody at a named protein rather than for co-occurrence. Captured names were resolved against UniProt nomenclature and each antigen adjudicated individually against the source text. Bibliographic records from PubMed and MeSH, U.S. National Library of Medicine; citation metrics from NIH iCite (Hutchins et al., PLoS Biology 2016). Titles link to PubMed; abstracts are not reproduced here. The NLM does not endorse this analysis.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.1

gnomAD pLI

0

gnomAD missense Z

0.45

DepMap mean gene effect

0

DepMap dependency class

none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• angiogenesis
• cell adhesion
• endothelial cell migration
• melanocyte apoptotic process
• melanocyte proliferation
• negative regulation of cell proliferation involved in contact inhibition
• negative regulation of extracellular matrix disassembly
• positive regulation of execution phase of apoptosis
• proteolysis
• proteolysis involved in protein catabolic process
• regulation of cell cycle
• regulation of collagen catabolic process
• regulation of fibrinolysis
• negative regulation of extracellular matrix organization

Molecular functions

• dipeptidyl-peptidase activity
• endopeptidase activity
• identical protein binding
• integrin binding
• peptidase activity
• protease binding
• protein homodimerization activity
• serine-type endopeptidase activity
• serine-type peptidase activity

Cellular components

• apical part of cell
• basal part of cell
• cell surface
• cytoplasm
• extracellular space
• focal adhesion
• lamellipodium
• lamellipodium membrane
• membrane
• plasma membrane
• ruffle membrane
• peptidase complex

Protein domainsUniProt · Pfam · InterPro

• Peptidase S9, prolyl oligopeptidase, catalytic domain
• Dipeptidylpeptidase IV, N-terminal domain
• Peptidase S9, serine active site
• Alpha/Beta hydrolase fold
• Serine protease S9B/DPPIV
• Prolyl oligopeptidase family
• Dipeptidyl peptidase IV (DPP IV) N-terminal region

KeywordsUniProt

• Angiogenesis
• Apoptosis
• Cell adhesion
• Cell junction
• Cell membrane
• Cell projection
• Cleavage on pair of basic residues
• Cytoplasm
• Disulfide bond
• Glycoprotein
• Hydrolase
• Membrane
• Protease
• Secreted
• Serine protease
• Signal-anchor
• Transmembrane
• Transmembrane helix

InteractionsUniProt · HPA

Protein binding partners of FAP in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• ITGA3
• ITGB1
• PLAUR