EPHA1

Protein identityUniProt · HPA

UniProt accession

P21709

Gene

EPHA1

Ensembl

ENSG00000146904

Chromosome

7

Canonical length

976 aa

Protein class

Cancer-related genes, Enzymes, Plasma proteins, Predicted membrane proteins

Quaternary structure

Homodimer

OverviewNCBI Gene

This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene is expressed in some human cancer cell lines and has been implicated in carcinogenesis. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

976 residues, UniProt reviewed canonical sequence.

>P21709|EPHA1
     1  MERRWPLGLG LVLLLCAPLP PGARAKEVTL MDTSKAQGEL GWLLDPPKDG WSEQQQILNG
    61  TPLYMYQDCP MQGRRDTDHW LRSNWIYRGE EASRVHVELQ FTVRDCKSFP GGAGPLGCKE
   121  TFNLLYMESD QDVGIQLRRP LFQKVTTVAA DQSFTIRDLV SGSVKLNVER CSLGRLTRRG
   181  LYLAFHNPGA CVALVSVRVF YQRCPETLNG LAQFPDTLPG PAGLVEVAGT CLPHARASPR
   241  PSGAPRMHCS PDGEWLVPVG RCHCEPGYEE GGSGEACVAC PSGSYRMDMD TPHCLTCPQQ
   301  STAESEGATI CTCESGHYRA PGEGPQVACT GPPSAPRNLS FSASGTQLSL RWEPPADTGG
   361  RQDVRYSVRC SQCQGTAQDG GPCQPCGVGV HFSPGARGLT TPAVHVNGLE PYANYTFNVE
   421  AQNGVSGLGS SGHASTSVSI SMGHAESLSG LSLRLVKKEP RQLELTWAGS RPRSPGANLT
   481  YELHVLNQDE ERYQMVLEPR VLLTELQPDT TYIVRVRMLT PLGPGPFSPD HEFRTSPPVS
   541  RGLTGGEIVA VIFGLLLGAA LLLGILVFRS RRAQRQRQQR QRDRATDVDR EDKLWLKPYV
   601  DLQAYEDPAQ GALDFTRELD PAWLMVDTVI GEGEFGEVYR GTLRLPSQDC KTVAIKTLKD
   661  TSPGGQWWNF LREATIMGQF SHPHILHLEG VVTKRKPIMI ITEFMENGAL DAFLREREDQ
   721  LVPGQLVAML QGIASGMNYL SNHNYVHRDL AARNILVNQN LCCKVSDFGL TRLLDDFDGT
   781  YETQGGKIPI RWTAPEAIAH RIFTTASDVW SFGIVMWEVL SFGDKPYGEM SNQEVMKSIE
   841  DGYRLPPPVD CPAPLYELMK NCWAYDRARR PHFQKLQAHL EQLLANPHSL RTIANFDPRM
   901  TLRLPSLSGS DGIPYRTVSE WLESIRMKRY ILHFHSAGLD TMECVLELTA EDLTQMGITL
   961  PGHQKRILCS IQGFKD

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against EPHA1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Cell surface

Secreted

No

Transmembrane segments

1

Mean surface accessibility (rSASA)

0.31

Highest tissue expression

194 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.05

gnomAD pLI

0

gnomAD missense Z

0.22

DepMap mean gene effect

0

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• angiogenesis
• cell surface receptor protein tyrosine kinase signaling pathway
• negative regulation of cell migration
• positive regulation of angiogenesis
• positive regulation of cell migration
• positive regulation of cell-matrix adhesion
• positive regulation of stress fiber assembly
• substrate adhesion-dependent cell spreading

Molecular functions

• ATP binding
• ephrin receptor activity
• fibronectin binding
• protein kinase binding
• transmembrane receptor protein tyrosine kinase activity
• transmembrane-ephrin receptor activity

Cellular components

• plasma membrane
• receptor complex

Protein domainsUniProt · Pfam · InterPro

• Protein kinase domain
• Ephrin, ligand binding domain
• Serine-threonine/tyrosine-protein kinase, catalytic domain
• Tyrosine-protein kinase, receptor class V, conserved site
• Sterile alpha motif domain
• Fibronectin type III
• Tyrosine-protein kinase, active site
• Galactose-binding-like domain superfamily
• Growth factor receptor cysteine-rich domain superfamily
• Protein kinase-like domain superfamily
• Tyrosine-protein kinase ephrin type A/B receptor-like
• Sterile alpha motif/pointed domain superfamily
• Immunoglobulin-like fold
• Ephrin receptor type-A /type-B
• Protein kinase, ATP binding site
• Tyrosine-protein kinase, catalytic domain
• Ephrin, transmembrane domain
• Fibronectin type III superfamily
• Ephrin receptor tyrosine kinases
• Fibronectin type III domain
• SAM domain (Sterile alpha motif)
• Ephrin receptor ligand binding domain
• Tyrosine-protein kinase ephrin type A/B receptor-like
• Protein tyrosine and serine/threonine kinase
• Ephrin type-A receptor 2 transmembrane domain
• Ephrin cysteine rich domain
• Ephrin type-A receptor 1, ligand binding domain

KeywordsUniProt

• Angiogenesis
• ATP-binding
• Cell adhesion
• Cell membrane
• Glycoprotein
• Kinase
• Membrane
• Nucleotide-binding
• Phosphoprotein
• Receptor
• Repeat
• Signal
• Transferase
• Transmembrane
• Transmembrane helix
• Tyrosine-protein kinase
• Ubl conjugation

InteractionsUniProt · HPA

Protein binding partners of EPHA1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• PTK2