EGLN2

Protein identityUniProt · HPA

UniProt accession

Q96KS0

Gene

EGLN2

Ensembl

ENSG00000269858

Chromosome

19

Canonical length

407 aa

Protein class

Enzymes, FDA approved drug targets, Plasma proteins, Predicted intracellular proteins

Subcellular location

Nucleoplasm

OverviewNCBI Gene

The hypoxia inducible factor (HIF) is a transcriptional complex that is involved in oxygen homeostasis. At normal oxygen levels, the alpha subunit of HIF is targeted for degration by prolyl hydroxylation. This gene encodes an enzyme responsible for this post-translational modification. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the upstream RAB4B (RAB4B, member RAS oncogene family) gene. [provided by RefSeq, Feb 2011]

Canonical amino-acid sequenceUniProt

407 residues, UniProt reviewed canonical sequence.

>Q96KS0|EGLN2
     1  MDSPCQPQPL SQALPQLPGS SSEPLEPEPG RARMGVESYL PCPLLPSYHC PGVPSEASAG
    61  SGTPRATATS TTASPLRDGF GGQDGGELRP LQSEGAAALV TKGCQRLAAQ GARPEAPKRK
   121  WAEDGGDAPS PSKRPWARQE NQEAEREGGM SCSCSSGSGE ASAGLMEEAL PSAPERLALD
   181  YIVPCMRYYG ICVKDSFLGA ALGGRVLAEV EALKRGGRLR DGQLVSQRAI PPRSIRGDQI
   241  AWVEGHEPGC RSIGALMAHV DAVIRHCAGR LGSYVINGRT KAMVACYPGN GLGYVRHVDN
   301  PHGDGRCITC IYYLNQNWDV KVHGGLLQIF PEGRPVVANI EPLFDRLLIF WSDRRNPHEV
   361  KPAYATRYAI TVWYFDAKER AAAKDKYQLA SGQKGVQVPV SQPPTPT

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against EGLN2 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.47

Highest tissue expression

224 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.45

gnomAD pLI

0.72

gnomAD missense Z

1.45

DepMap mean gene effect

-0.16

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• cell redox homeostasis
• cellular response to hypoxia
• estrogen receptor signaling pathway
• peptidyl-proline hydroxylation to 4-hydroxy-L-proline
• positive regulation of protein catabolic process
• regulation of cell growth
• regulation of neuron apoptotic process
• response to hypoxia

Molecular functions

• 2-oxoglutarate-dependent dioxygenase activity
• ferrous iron binding
• hypoxia-inducible factor-proline dioxygenase activity
• L-ascorbic acid binding
• oxygen sensor activity
• peptidyl-proline 4-dioxygenase activity

Cellular components

• cytoplasm
• nucleoplasm
• nucleus

Protein domainsUniProt · Pfam · InterPro

• Oxoglutarate/iron-dependent dioxygenase domain
• Prolyl 4-hydroxylase, alpha subunit
• Prolyl 4-hydroxylase alpha subunit, Fe(2+) 2OG dioxygenase domain
• Hypoxia-inducible factor prolyl hydroxylases
• 2OG-Fe(II) oxygenase superfamily

KeywordsUniProt

• Alternative initiation
• Dioxygenase
• Iron
• Metal-binding
• Nucleus
• Oxidoreductase
• Phosphoprotein
• Ubl conjugation
• Vitamin C

InteractionsUniProt · HPA

Protein binding partners of EGLN2 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• AJUBA
• LIMD1
• WTIP
• SIAH2
• HIF1A