ECH1

Protein identityUniProt · HPA

UniProt accession

Q13011

Gene

ECH1

Ensembl

ENSG00000104823

Chromosome

19

Canonical length

328 aa

Protein class

Metabolic proteins, Predicted intracellular proteins

Subcellular location

Mitochondria

Quaternary structure

Homohexamer

OverviewNCBI Gene

This gene encodes a member of the hydratase/isomerase superfamily. The gene product shows high sequence similarity to enoyl-coenzyme A (CoA) hydratases of several species, particularly within a conserved domain characteristic of these proteins. The encoded protein, which contains a C-terminal peroxisomal targeting sequence, localizes to the peroxisome. The rat ortholog, which localizes to the matrix of both the peroxisome and mitochondria, can isomerize 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA, indicating that it is a delta3,5-delta2,4-dienoyl-CoA isomerase. This enzyme functions in the auxiliary step of the fatty acid beta-oxidation pathway. Expression of the rat gene is induced by peroxisome proliferators. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

328 residues, UniProt reviewed canonical sequence.

>Q13011|ECH1
     1  MAAGIVASRR LRDLLTRRLT GSNYPGLSIS LRLTGSSAQE EASGVALGEA PDHSYESLRV
    61  TSAQKHVLHV QLNRPNKRNA MNKVFWREMV ECFNKISRDA DCRAVVISGA GKMFTAGIDL
   121  MDMASDILQP KGDDVARISW YLRDIITRYQ ETFNVIERCP KPVIAAVHGG CIGGGVDLVT
   181  ACDIRYCAQD AFFQVKEVDV GLAADVGTLQ RLPKVIGNQS LVNELAFTAR KMMADEALGS
   241  GLVSRVFPDK EVMLDAALAL AAEISSKSPV AVQSTKVNLL YSRDHSVAES LNYVASWNMS
   301  MLQTQDLVKS VQATTENKEL KTVTFSKL

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ECH1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.33

Highest tissue expression

904 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.13

gnomAD pLI

0

gnomAD missense Z

0.04

DepMap mean gene effect

-0.1

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 8% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• fatty acid beta-oxidation

Molecular functions

• delta(3
• 5)-delta(2
• 4)-dienoyl-CoA isomerase activity

Cellular components

• cytosol
• extracellular exosome
• membrane
• mitochondrial matrix
• mitochondrion
• peroxisomal matrix
• peroxisome

Protein domainsUniProt · Pfam · InterPro

• Enoyl-CoA hydratase/isomerase-like domain
• Enoyl-CoA hydratase, C-terminal
• Enoyl-CoA hydratase/isomerase, conserved site
• ClpP/crotonase-like domain superfamily
• Enoyl-CoA hydratase/isomerase
• Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase Ech1-like

KeywordsUniProt

• Acetylation
• Fatty acid metabolism
• Isomerase
• Lipid metabolism
• Mitochondrion
• Peroxisome
• Phosphoprotein
• Transit peptide

InteractionsUniProt · HPA

Protein binding partners of ECH1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• ECI2
• EEF1G
• HTT
• STAT3
• TRAF1