ECI2

Protein identityUniProt · HPA

UniProt accession

O75521

Gene

ECI2

Ensembl

ENSG00000198721

Chromosome

6

Canonical length

394 aa

Protein class

Enzymes, Metabolic proteins, Predicted intracellular proteins

Subcellular location

Peroxisomes,Mitochondria

OverviewNCBI Gene

This gene encodes a member of the hydratase/isomerase superfamily. The protein encoded is a key mitochondrial enzyme involved in beta-oxidation of unsaturated fatty acids. It catalyzes the transformation of 3-cis and 3-trans-enoyl-CoA esters arising during the stepwise degradation of cis-, mono-, and polyunsaturated fatty acids to the 2-trans-enoyl-CoA intermediates. Alternatively spliced transcript variants have been described. [provided by RefSeq, Aug 2011]

Canonical amino-acid sequenceUniProt

394 residues, UniProt reviewed canonical sequence.

>O75521|ECI2
     1  MAMAYLAWRL ARRSCPSSLQ VTSFPVVQLH MNRTAMRASQ KDFENSMNQV KLLKKDPGNE
    61  VKLKLYALYK QATEGPCNMP KPGVFDLINK AKWDAWNALG SLPKEAARQN YVDLVSSLSP
   121  SLESSSQVEP GTDRKSTGFE TLVVTSEDGI TKIMFNRPKK KNAINTEMYH EIMRALKAAS
   181  KDDSIITVLT GNGDYYSSGN DLTNFTDIPP GGVEEKAKNN AVLLREFVGC FIDFPKPLIA
   241  VVNGPAVGIS VTLLGLFDAV YASDRATFHT PFSHLGQSPE GCSSYTFPKI MSPAKATEML
   301  IFGKKLTAGE ACAQGLVTEV FPDSTFQKEV WTRLKAFAKL PPNALRISKE VIRKREREKL
   361  HAVNAEECNV LQGRWLSDEC TNAVVNFLSR KSKL

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ECI2 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.33

Highest tissue expression

395 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.23

gnomAD pLI

0

gnomAD missense Z

-0.01

DepMap mean gene effect

-0.06

DepMap dependency class

none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• fatty acid beta-oxidation
• fatty acid catabolic process

Molecular functions

• delta(3)-delta(2)-enoyl-CoA isomerase activity
• fatty-acyl-CoA binding

Cellular components

• cytosol
• membrane
• mitochondrion
• peroxisomal matrix
• peroxisome

Protein domainsUniProt · Pfam · InterPro

• Acyl-CoA-binding protein, ACBP
• Enoyl-CoA hydratase/isomerase-like domain
• FERM/acyl-CoA-binding protein superfamily
• Enoyl-CoA hydratase, C-terminal
• Acyl-CoA-binding protein, ACBP, conserved site
• ClpP/crotonase-like domain superfamily
• Acyl-CoA binding protein superfamily
• Enoyl-CoA hydratase/isomerase and chromodomain-containing protein
• Enoyl-CoA hydratase/isomerase
• Acyl CoA binding protein

KeywordsUniProt

• Acetylation
• Isomerase
• Mitochondrion
• Peroxisome
• Phosphoprotein
• Transit peptide

InteractionsUniProt · HPA

Protein binding partners of ECI2 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• ECH1