E2F5

Protein identityUniProt · HPA

UniProt accession

Q15329

Gene

E2F5

Ensembl

ENSG00000133740

Chromosome

8

Canonical length

346 aa

Protein class

Cancer-related genes, Predicted intracellular proteins, Predicted membrane proteins, Transcription factors

Subcellular location

Nucleoplasm,Nucleoli,Vesicles

OverviewNCBI Gene

The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionarily conserved domains that are present in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein is differentially phosphorylated and is expressed in a wide variety of human tissues. It has higher identity to E2F4 than to other family members. Both this protein and E2F4 interact with tumor suppressor proteins p130 and p107, but not with pRB. Alternative splicing results in multiple variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

346 residues, UniProt reviewed canonical sequence.

>Q15329|E2F5
     1  MAAAEPASSG QQAPAGQGQG QRPPPQPPQA QAPQPPPPPQ LGGAGGGSSR HEKSLGLLTT
    61  KFVSLLQEAK DGVLDLKAAA DTLAVRQKRR IYDITNVLEG IDLIEKKSKN SIQWKGVGAG
   121  CNTKEVIDRL RYLKAEIEDL ELKERELDQQ KLWLQQSIKN VMDDSINNRF SYVTHEDICN
   181  CFNGDTLLAI QAPSGTQLEV PIPEMGQNGQ KKYQINLKSH SGPIHVLLIN KESSSSKPVV
   241  FPVPPPDDLT QPSSQSLTPV TPQKSSMATQ NLPEQHVSER SQALQQTSAT DISSAGSISG
   301  DIIDELMSSD VFPLLRLSPT PADDYNFNLD DNEGVCDLFD VQILNY

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against E2F5 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.54

Highest tissue expression

28 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.36

gnomAD pLI

0.94

gnomAD missense Z

0.86

DepMap mean gene effect

0.06

DepMap dependency class

none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 11% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• animal organ morphogenesis
• cell projection organization
• regulation of transcription by RNA polymerase II

Molecular functions

• DNA-binding transcription activator activity
• DNA-binding transcription factor activity
• DNA-binding transcription factor activity, RNA polymerase II-specific
• protein dimerization activity
• RNA polymerase II cis-regulatory region sequence-specific DNA binding

Cellular components

• chromatin
• cytoplasm
• fibrillar center
• nucleolus
• nucleoplasm
• RNA polymerase II transcription regulator complex

Protein domainsUniProt · Pfam · InterPro

• E2F/DP family, winged-helix DNA-binding domain
• E2F Family
• E2F transcription factor, CC-MB domain
• Winged helix-like DNA-binding domain superfamily
• Winged helix DNA-binding domain superfamily
• E2F-DP heterodimerization region
• E2F/DP family winged-helix DNA-binding domain
• E2F transcription factor CC-MB domain

KeywordsUniProt

• Activator
• Cilium biogenesis/degradation
• DNA-binding
• Nucleus
• Transcription
• Transcription regulation