Seroatlas · Human Serome Atlas

DPPA4

Developmental pluripotency-associated protein 4

Also known as: DPPA4_HUMAN, FLJ10713

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q7L190
Gene: DPPA4
Ensembl: ENSG00000121570
Chromosome: 3
Canonical length: 304 aa
Protein class: Predicted intracellular proteins
Subcellular location: Nucleoplasm,Nucleoli,Nucleoli rim,Mitotic chromosome,Cytosol

OverviewNCBI Gene

This gene encodes a nuclear factor that is involved in the maintenance of pluripotency in stem cells and essential for embryogenesis. The encoded protein has a scaffold-attachment factor A/B, acinus and PIAS (SAP) domain that binds DNA and is thought to modify chromatin. Mice with a homozygous knockout of the orthologous gene die during late embryonic development or within hours after birth. Knockout embryos are normal in size at embryonic day 18.5 but exhibit skeletal and lung tissue abnormalities. This gene, when mutated, is highly expressed in embryonal carcinomas, pluripotent germ cell tumors, and other cancers and is thought to play an important role in tumor progression. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2017]

Canonical amino-acid sequenceUniProt

304 residues, UniProt reviewed canonical sequence.

>Q7L190|DPPA4
     1  MLRGSASSTS MEKAKGKEWT STEKSREEDQ QASNQPNSIA LPGTSAKRTK EKMSIKGSKV
    61  LCPKKKAEHT DNPRPQKKIP IPPLPSKLPP VNLIHRDILR AWCQQLKLSS KGQKLDAYKR
   121  LCAFAYPNQK DFPSTAKEAK IRKSLQKKLK VEKGETSLQS SETHPPEVAL PPVGEPPALE
   181  NSTALLEGVN TVVVTTSAPE ALLASWARIS ARARTPEAVE SPQEASGVRW CVVHGKSLPA
   241  DTDGWVHLQF HAGQAWVPEK QEGRVSALFL LPASNFPPPH LEDNMLCPKC VHRNKVLIKS
   301  LQWE

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against DPPA4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.54
Highest tissue expression: 12 nTPM

Expression across tissuesHPA

Tissue

thymus: 12 nTPM
bone marrow: 4.5 nTPM
testis: 3.9 nTPM
placenta: 2.1 nTPM
pituitary gland: 0.7 nTPM
liver: 0.6 nTPM

Single-cell type

hematopoietic stem cells: 140 nCPM
megakaryocyte progenitors: 82 nCPM
megakaryocyte-erythroid progenitors: 65 nCPM
thymocytes: 53 nCPM
megakaryocytes: 37 nCPM
cytotrophoblasts: 31 nCPM

Immune cell

plasmacytoid DC: 7.5 nTPM
naive B-cell: 0.6 nTPM
NK-cell: 0.5 nTPM
myeloid DC: 0.1 nTPM
naive CD4 T-cell: 0.1 nTPM
naive CD8 T-cell: 0.1 nTPM

Brain region

basal ganglia: 0.3 nTPM
cerebellum: 0.3 nTPM
medulla oblongata: 0.3 nTPM
pons: 0.3 nTPM
cerebral cortex: 0.2 nTPM
hypothalamus: 0.2 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.95
gnomAD pLI: 0
gnomAD missense Z: 0.34
DepMap mean gene effect: 0.05
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

chromatin binding

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of DPPA4 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads DPPA4 as an antibody target. Whether an autoantibody or antibody against DPPA4 could matter depends on whether native DPPA4 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

DPPA4 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label DPPA4 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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