Seroatlas · Human Serome Atlas

DNTT

DNA nucleotidylexotransferase

Also known as: TDT, TDT_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: P04053
Gene: DNTT
Ensembl: ENSG00000107447
Chromosome: 10
Canonical length: 509 aa
Protein class: Enzymes, Plasma proteins, Predicted intracellular proteins
Subcellular location: Nucleoplasm,Cytosol

OverviewNCBI Gene

This gene is a member of the DNA polymerase type-X family and encodes a template-independent DNA polymerase that catalyzes the addition of deoxynucleotides to the 3'-hydroxyl terminus of oligonucleotide primers. In vivo, the encoded protein is expressed in a restricted population of normal and malignant pre-B and pre-T lymphocytes during early differentiation, where it generates antigen receptor diversity by synthesizing non-germ line elements (N-regions) at the junctions of rearranged Ig heavy chain and T cell receptor gene segments. Alternatively spliced transcript variants encoding different isoforms of this gene have been described. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

509 residues, UniProt reviewed canonical sequence.

>P04053|DNTT
     1  MDPPRASHLS PRKKRPRQTG ALMASSPQDI KFQDLVVFIL EKKMGTTRRA FLMELARRKG
    61  FRVENELSDS VTHIVAENNS GSDVLEWLQA QKVQVSSQPE LLDVSWLIEC IRAGKPVEMT
   121  GKHQLVVRRD YSDSTNPGPP KTPPIAVQKI SQYACQRRTT LNNCNQIFTD AFDILAENCE
   181  FRENEDSCVT FMRAASVLKS LPFTIISMKD TEGIPCLGSK VKGIIEEIIE DGESSEVKAV
   241  LNDERYQSFK LFTSVFGVGL KTSEKWFRMG FRTLSKVRSD KSLKFTRMQK AGFLYYEDLV
   301  SCVTRAEAEA VSVLVKEAVW AFLPDAFVTM TGGFRRGKKM GHDVDFLITS PGSTEDEEQL
   361  LQKVMNLWEK KGLLLYYDLV ESTFEKLRLP SRKVDALDHF QKCFLIFKLP RQRVDSDQSS
   421  WQEGKTWKAI RVDLVLCPYE RRAFALLGWT GSRQFERDLR RYATHERKMI LDNHALYDKT
   481  KRIFLKAESE EEIFAHLGLD YIEPWERNA

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against DNTT can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.31
Highest tissue expression: 306 nTPM

Expression across tissuesHPA

Tissue

thymus: 306 nTPM
bone marrow: 26 nTPM
tonsil: 2.1 nTPM
testis: 0.7 nTPM
thyroid gland: 0.4 nTPM
lymph node: 0.3 nTPM

Single-cell type

thymocytes: 160 nCPM
early spermatids: 19 nCPM
late spermatids: 14 nCPM
late primary spermatocytes: 0.8 nCPM
corticotrophs: 0.6 nCPM
undifferentiated spermatogonia: 0.6 nCPM

Immune cell

naive CD4 T-cell: 1.3 nTPM
naive B-cell: 0.7 nTPM
total PBMC: 0.1 nTPM
basophil: 0 nTPM
classical monocyte: 0 nTPM
eosinophil: 0 nTPM

Brain region

white matter: 0.7 nTPM
basal ganglia: 0.3 nTPM
cerebral cortex: 0.2 nTPM
hypothalamus: 0.2 nTPM
thalamus: 0.2 nTPM
amygdala: 0.1 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.01
gnomAD pLI: 0
gnomAD missense Z: -0.47
DepMap mean gene effect: 0
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 2% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of DNTT in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads DNTT as an antibody target. Whether an autoantibody or antibody against DNTT could matter depends on whether native DNTT is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

DNTT is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label DNTT as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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