Seroatlas · Human Serome Atlas

DNAJB8

DnaJ homolog subfamily B member 8

Also known as: CT156, DNJB8_HUMAN, MGC33884

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q8NHS0
Gene: DNAJB8
Ensembl: ENSG00000179407
Chromosome: 3
Canonical length: 232 aa
Protein class: Predicted intracellular proteins

OverviewNCBI Gene

The protein encoded by this gene belongs to the DNAJ/HSP40 family of proteins that regulate chaperone activity. This family member suppresses aggregation and toxicity of polyglutamine proteins, and the C-terminal tail is essential for this activity. It has been implicated as a cancer-testis antigen and as a cancer stem-like cell antigen involved in renal cell carcinoma. [provided by RefSeq, Jun 2012]

Canonical amino-acid sequenceUniProt

232 residues, UniProt reviewed canonical sequence.

>Q8NHS0|DNAJB8
     1  MANYYEVLGV QASASPEDIK KAYRKLALRW HPDKNPDNKE EAEKKFKLVS EAYEVLSDSK
    61  KRSLYDRAGC DSWRAGGGAS TPYHSPFDTG YTFRNPEDIF REFFGGLDPF SFEFWDSPFN
   121  SDRGGRGHGL RGAFSAGFGE FPAFMEAFSS FNMLGCSGGS HTTFSSTSFG GSSSGSSGFK
   181  SVMSSTEMIN GHKVTTKRIV ENGQERVEVE EDGQLKSVTV NGKEQLKWMD SK

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against DNAJB8 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.46
Highest tissue expression: 58 nTPM

Expression across tissuesHPA

Tissue

testis: 58 nTPM
adipose tissue: 0 nTPM
adrenal gland: 0 nTPM
amygdala: 0 nTPM
appendix: 0 nTPM
basal ganglia: 0 nTPM

Single-cell type

late spermatids: 6,976 nCPM
early spermatids: 1,358 nCPM
late primary spermatocytes: 202 nCPM
sertoli cells: 13 nCPM
leydig cells: 6.4 nCPM
undifferentiated spermatogonia: 5.3 nCPM

Immune cell

basophil: 0 nTPM
classical monocyte: 0 nTPM
eosinophil: 0 nTPM
gdT-cell: 0 nTPM
intermediate monocyte: 0 nTPM
MAIT T-cell: 0 nTPM

Brain region

cerebellum: 0.1 nTPM
cerebral cortex: 0.1 nTPM
amygdala: 0 nTPM
basal ganglia: 0 nTPM
choroid plexus: 0 nTPM
hippocampal formation: 0 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.3
gnomAD pLI: 0.1
gnomAD missense Z: -0.52
DepMap mean gene effect: -0.07
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Chaperone

InteractionsUniProt · HPA

Protein binding partners of DNAJB8 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads DNAJB8 as an antibody target. Whether an autoantibody or antibody against DNAJB8 could matter depends on whether native DNAJB8 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

DNAJB8 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label DNAJB8 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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