Seroatlas · Human Serome Atlas

DNAI3

Dynein axonemal intermediate chain 3

Also known as: DIC3, DNAI3_HUMAN, FLJ30067, NYD-SP29, WDR63

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q8IWG1
Gene: DNAI3
Ensembl: ENSG00000162643
Chromosome: 1
Canonical length: 891 aa
Protein class: Disease related genes, Predicted intracellular proteins
Subcellular location: Perinuclear theca,Calyx,Connecting piece,Mid piece

OverviewNCBI Gene

Enables Arp2/3 complex binding activity. Involved in negative regulation of Arp2/3 complex-mediated actin nucleation and negative regulation of cell migration. Located in cytoplasm. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

891 residues, UniProt reviewed canonical sequence.

>Q8IWG1|DNAI3
     1  MAPKQKKKTS RGKKRLKPVL AASEDMEPVN MESMGHPEIY PLVLTTKTQE IFNCRIDEDV
    61  TDEQPYKLIN KEDIFEDLRN RAAVSDFHPV KKIVQEYPGN ELLLVYDKDF KYGLNFYLIA
   121  TEEGKENYLN PPEVPEEQEE YKEHIPEDVY IYKPPVSKPW VSLGSEKEIE EESVTESTKQ
   181  ITYMISRKRS EFGAPIKFSD QNASSVKDAY IECTAYPDKN FTLKQLEKDV GMQVIPQIKD
   241  ISTQTKWTYP KNATTQYYPR EFSEEEKETL KQSKPLVDFL NNASISVEIA LQQNEIMNTF
   301  IDDWKYLAEE EGTFGDKTDT HLKEYQSFTD LHSPTEKMIT CVSWHPTIYG LIAVSVAVRL
   361  SFEDRVHFSG KLLLQPSLIL FWSFSDPIHP QLMLESPDDI FCFKFCPSDP NIIAGGCING
   421  QIVMWDITAH ADRIENIKAG GSRSKRATLK PMFLLEPESN KEAMYIRHCA VSSIENGHKK
   481  VITDIHWLSD TFEINRMGSV FENRSGICCQ LVTCSADCTI CFWDIRPQKP LTPQTTEKKK
   541  EESIEIPFDV PSTFLHLDLS WKPLTKVRLS KGETSLDHCP TKISLNEDHL LCKTQDKMLA
   601  QSKTEKAEEM NPYHNLESGM ANLLKPIDDF CTKFFVGTEE GEVIYTDWKM EKDPETGRLM
   661  SKKPVSHHTI HDGTVHTIQR SPFYNDIILT VGGWNVAIWK EGVMTGPLLQ SCCAPKRYTS
   721  GHWSLTRPGV FYIGREDGYI DIWDLLEKTH EPAQSQNICI TMITYIKPWI FSSKQQFIAT
   781  ADYYGTLHIL EIPWTLSRPS TNEMASVNHY FEREVKHLEY VEQRKKIREQ EKKEMELEMA
   841  KKKVKTYQKS KEQMQAELKM DYESYLELEK TVLINLGLIK VTEKGSYMEV M

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against DNAI3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.39
Highest tissue expression: 40 nTPM

Expression across tissuesHPA

Tissue

choroid plexus: 40 nTPM
adrenal gland: 21 nTPM
testis: 18 nTPM
fallopian tube: 14 nTPM
parathyroid gland: 5.3 nTPM
epididymis: 3.9 nTPM

Single-cell type

ependymal cells: 451 nCPM
melanocytes: 364 nCPM
respiratory ciliated cells: 286 nCPM
fallopian tube ciliated cells: 282 nCPM
epididymal efferent duct ciliated cells: 219 nCPM
endometrial ciliated cells: 213 nCPM

Immune cell

gdT-cell: 0.4 nTPM
naive CD8 T-cell: 0.3 nTPM
MAIT T-cell: 0.2 nTPM
memory CD4 T-cell: 0.2 nTPM
memory CD8 T-cell: 0.1 nTPM
naive CD4 T-cell: 0.1 nTPM

Brain region

choroid plexus: 50 nTPM
midbrain: 22 nTPM
medulla oblongata: 18 nTPM
spinal cord: 14 nTPM
white matter: 8.2 nTPM
pons: 7.2 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about DNAI3.

Disease | GeneticClinVar

1 pathogenic / likely-pathogenic of 142 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

Occipital encephalocele

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.76
gnomAD pLI: 0
DepMap mean gene effect: -0.22
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of DNAI3 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads DNAI3 as an antibody target. Whether an autoantibody or antibody against DNAI3 could matter depends on whether native DNAI3 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

DNAI3 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label DNAI3 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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