DHX35

Protein identityUniProt · HPA

UniProt accession

Q9H5Z1

Gene

DHX35

Ensembl

ENSG00000101452

Chromosome

20

Canonical length

703 aa

Protein class

Enzymes, Plasma proteins, Predicted intracellular proteins

Subcellular location

Nucleoplasm,Nuclear bodies,Centrosome

OverviewNCBI Gene

DEAD box proteins characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of the DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The function of this gene product which is a member of this family, has not been determined. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jun 2010]

Canonical amino-acid sequenceUniProt

703 residues, UniProt reviewed canonical sequence.

>Q9H5Z1|DHX35
     1  MAAPVGPVKF WRPGTEGPGV SISEERQSLA ENSGTTVVYN PYAALSIEQQ RQKLPVFKLR
    61  NHILYLIENY QTVVIVGETG CGKSTQIPQY LAEAGWTAEG RVVGVTQPRR VAAVTVAGRV
   121  AEERGAVLGH EVGYCIRFDD CTDQLATRIK FLTDGMLVRE MMVDPLLTKY SVIMLDEAHE
   181  RTLYTDIAIG LLKKIQKKRG DLRLIVASAT LDADKFRDFF NQNETSDPAR DTCVILTVEG
   241  RTFPVDIFYL QSPVPDYIKS TVETVVKIHQ TEGDGDVLAF LTGQEEVETV VSMLIEQARA
   301  LARTGMKRHL RVLPMYAGLP SFEQMKVFER VSRSVRKVIV ATNVAETSIT ISGIVYVIDC
   361  GFVKLRAYNP RTAIECLVVV PVSQASANQR AGRGGRSRSG KCYRLYTEEA FDKLPQSTVP
   421  EMQRSNLAPV ILQLKALGID NVLRFHFMSP PPAQSMVQAL ELLYALGGLD KDCRLTEPLG
   481  MRIAEFPLNP MFAKMLLESG NFGCSQEILS IAAMMQIQNI FVVPPNQKSH AIRVHRKFAV
   541  EEGDHLTMLN IYEAFIKHNK DSKWCQEHFL NYKGLVRAAT VREQLKKLLV KFQVPRKSSE
   601  GDPDLVLRCI VSGFFANAAR FHSTGAYRTI RDDHELHIHP ASVLYAEKPP RWVIYNEVIQ
   661  TSKYYMRDVT AIESAWLLEL APHFYQQGTH LSLKAKRAKV QDP

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against DHX35 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.25

Highest tissue expression

17 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.13

gnomAD pLI

0

gnomAD missense Z

0.48

DepMap mean gene effect

-0.29

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 14% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• in utero embryonic development
• mRNA splicing, via spliceosome

Molecular functions

• ATP binding
• ATP hydrolysis activity
• helicase activity
• RNA binding
• RNA helicase activity

Cellular components

• catalytic step 2 spliceosome
• nucleoplasm

Protein domainsUniProt · Pfam · InterPro

• Helicase, C-terminal domain-like
• DNA/RNA helicase, ATP-dependent, DEAH-box type, conserved site
• Helicase-associated domain
• DEAD-box helicase, OB fold
• Helicase superfamily 1/2, ATP-binding domain
• P-loop containing nucleoside triphosphate hydrolase
• Helicase associated domain (HA2), winged-helix domain
• Helicase conserved C-terminal domain
• Helicase associated domain (HA2), winged-helix
• Oligonucleotide/oligosaccharide-binding (OB)-fold
• Helicase associated domain (HA2), ratchet-like

KeywordsUniProt

• ATP-binding
• Helicase
• Hydrolase
• mRNA processing
• mRNA splicing
• Nucleotide-binding
• Spliceosome