DFFA

Protein identityUniProt · HPA

UniProt accession

O00273

Gene

DFFA

Ensembl

ENSG00000160049

Chromosome

1

Canonical length

331 aa

Protein class

Plasma proteins, Predicted intracellular proteins

Subcellular location

Plasma membrane,Cytosol

OverviewNCBI Gene

Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

331 residues, UniProt reviewed canonical sequence.

>O00273|DFFA
     1  MEVTGDAGVP ESGEIRTLKP CLLRRNYSRE QHGVAASCLE DLRSKACDIL AIDKSLTPVT
    61  LVLAEDGTIV DDDDYFLCLP SNTKFVALAS NEKWAYNNSD GGTAWISQES FDVDETDSGA
   121  GLKWKNVARQ LKEDLSSIIL LSEEDLQMLV DAPCSDLAQE LRQSCATVQR LQHTLQQVLD
   181  QREEVRQSKQ LLQLYLQALE KEGSLLSKQE ESKAAFGEEV DAVDTGISRE TSSDVALASH
   241  ILTALREKQA PELSLSSQDL ELVTKEDPKA LAVALNWDIK KTETVQEACE RELALRLQQT
   301  QSLHSLRSIS ASKASPPGDL QNPKRARQDP T

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against DFFA can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.42

Highest tissue expression

19 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.16

gnomAD pLI

0

gnomAD missense Z

0.32

DepMap mean gene effect

0.05

DepMap dependency class

none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• apoptotic DNA fragmentation
• apoptotic process
• negative regulation of apoptotic DNA fragmentation
• negative regulation of execution phase of apoptosis
• positive regulation of apoptotic process
• thymocyte apoptotic process

Molecular functions

• protein folding chaperone
• deoxyribonuclease inhibitor activity

Cellular components

• chromatin
• cytosol
• nucleoplasm
• nucleus
• plasma membrane
• protein-containing complex

Protein domainsUniProt · Pfam · InterPro

• CIDE-N domain
• CIDE-N domain
• DNA fragmentation factor 45kDa, middle domain
• DNA fragmentation factor 45
• DNA fragmentation factor 45kDa, C-terminal
• DNA Fragmentation factor 45kDa, C terminal domain

KeywordsUniProt

• Acetylation
• Apoptosis
• Cytoplasm
• Phosphoprotein

InteractionsUniProt · HPA

Protein binding partners of DFFA in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• CIDEB
• DFFB
• HSPB1
• YWHAZ