Seroatlas · Human Serome Atlas

DENND1B

DENN domain-containing protein 1B

Also known as: C1orf218, DEN1B_HUMAN, FAM31B, FLJ20054, MGC27044

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q6P3S1
Gene
DENND1B
Ensembl
ENSG00000213047
Chromosome
1
Canonical length
775 aa
Protein class
Disease related genes, Predicted intracellular proteins, Predicted membrane proteins
Subcellular location
Nuclear speckles,Cytosol

OverviewNCBI Gene

Clathrin (see MIM 118955)-mediated endocytosis is a major mechanism for internalization of proteins and lipids. Members of the connecdenn family, such as DENND1B, function as guanine nucleotide exchange factors (GEFs) for the early endosomal small GTPase RAB35 (MIM 604199) and bind to clathrin and clathrin adaptor protein-2 (AP2; see MIM 601024). Thus, connecdenns link RAB35 activation with the clathrin machinery (Marat and McPherson, 2010 [PubMed 20154091]).[supplied by OMIM, Nov 2010]

Canonical amino-acid sequenceUniProt

775 residues, UniProt reviewed canonical sequence.

>Q6P3S1|DENND1B
     1  MDCRTKANPD RTFDLVLKVK CHASENEDPV VLWKFPEDFG DQEILQSVPK FCFPFDVERV
    61  SQNQVGQHFT FVLTDIESKQ RFGFCRLTSG GTICLCILSY LPWFEVYYKL LNTLADYLAK
   121  ELENDLNETL RSLYNHPVPK ANTPVNLSVN QEIFIACEQV LKDQPALVPH SYFIAPDVTG
   181  LPTIPESRNL TEYFVAVDVN NMLQLYASML HERRIVIISS KLSTLTACIH GSAALLYPMY
   241  WQHIYIPVLP PHLLDYCCAP MPYLIGIHSS LIERVKNKSL EDVVMLNVDT NTLESPFSDL
   301  NNLPSDVVSA LKNKLKKQST ATGDGVARAF LRAQAALFGS YRDALRYKPG EPITFCEESF
   361  VKHRSSVMKQ FLETAINLQL FKQFIDGRLA KLNAGRGFSD VFEEEITSGG FCGGNPRSYQ
   421  QWVHTVKKGG ALFNTAMTKA TPAVRTAYKF AKNHAKLGLK EVKSKLKHKE NEEDYGTCSS
   481  SVQYTPVYKL HNEKGGNSEK RKLAQARLKR PLKSLDGALY DDEDDDDIER ASKLSSEDGE
   541  EASAYLYESD DSVETRVKTP YSGEMDLLGE ILDTLSTHSS DQGKLAAAKS LDFFRSMDDI
   601  DYKPTNKSNA PSENNLAFLC GGSGDQAEWN LGQDDSALHG KHLPPSPRKR VSSSGLTDSL
   661  FILKEENSNK HLGADNVSDP TSGLDFQLTS PEVSQTDKGK TEKRETLSQI SDDLLIPGLG
   721  RHSSTFVPWE KEGKEAKETS EDIGLLHEVV SLCHMTSDFQ QSLNISDKNT NGNQT

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against DENND1B can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.44
Highest tissue expression
6.5 nTPM

Expression across tissuesHPA

Tissue

  • small intestine: 6.5 nTPM
  • thymus: 6.2 nTPM
  • tonsil: 6.1 nTPM
  • lymph node: 6 nTPM
  • colon: 5.6 nTPM
  • thyroid gland: 5.6 nTPM

Single-cell type

  • ocular epithelial cells: 540 nCPM
  • neutrophil progenitors: 366 nCPM
  • cdc: 345 nCPM
  • plasma cells: 301 nCPM
  • prostatic hillock cells: 280 nCPM
  • enterocytes: 274 nCPM

Immune cell

  • NK-cell: 2.7 nTPM
  • basophil: 2.3 nTPM
  • T-reg: 1.8 nTPM
  • memory CD8 T-cell: 1.4 nTPM
  • memory B-cell: 1.2 nTPM
  • eosinophil: 1.1 nTPM

Brain region

  • basal ganglia: 25 nTPM
  • midbrain: 19 nTPM
  • thalamus: 17 nTPM
  • hypothalamus: 17 nTPM
  • cerebellum: 16 nTPM
  • pons: 15 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about DENND1B.

Disease | AllUniProt

Conditions DENND1B is implicated in, by any mechanism.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.49
gnomAD pLI
0.2
gnomAD missense Z
0.33
DepMap mean gene effect
0.08
DepMap dependency class
none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 8% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of DENND1B in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads DENND1B as an antibody target. Whether an autoantibody or antibody against DENND1B could matter depends on whether native DENND1B is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

DENND1B is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label DENND1B as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/DENND1B. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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