DAGLA

Protein identityUniProt · HPA

UniProt accession

Q9Y4D2

Gene

DAGLA

Ensembl

ENSG00000134780

Chromosome

11

Canonical length

1042 aa

Protein class

Disease related genes, Enzymes, Metabolic proteins, Potential drug targets, Predicted membrane proteins

OverviewNCBI Gene

This gene encodes a diacylglycerol lipase. The encoded enzyme is involved in the biosynthesis of the endocannabinoid 2-arachidonoyl-glycerol.[provided by RefSeq, Nov 2010]

Canonical amino-acid sequenceUniProt

1042 residues, UniProt reviewed canonical sequence.

>Q9Y4D2|DAGLA
     1  MPGIVVFRRR WSVGSDDLVL PAIFLFLLHT TWFVILSVVL FGLVYNPHEA CSLNLVDHGR
    61  GYLGILLSCM IAEMAIIWLS MRGGILYTEP RDSMQYVLYV RLAILVIEFI YAIVGIVWLT
   121  QYYTSCNDLT AKNVTLGMVV CNWVVILSVC ITVLCVFDPT GRTFVKLRAT KRRQRNLRTY
   181  NLRHRLEEGQ ATSWSRRLKV FLCCTRTKDS QSDAYSEIAY LFAEFFRDLD IVPSDIIAGL
   241  VLLRQRQRAK RNAVLDEANN DILAFLSGMP VTRNTKYLDL KNSQEMLRYK EVCYYMLFAL
   301  AAYGWPMYLM RKPACGLCQL ARSCSCCLCP ARPRFAPGVT IEEDNCCGCN AIAIRRHFLD
   361  ENMTAVDIVY TSCHDAVYET PFYVAVDHDK KKVVISIRGT LSPKDALTDL TGDAERLPVE
   421  GHHGTWLGHK GMVLSAEYIK KKLEQEMVLS QAFGRDLGRG TKHYGLIVVG HSLGAGTAAI
   481  LSFLLRPQYP TLKCFAYSPP GGLLSEDAME YSKEFVTAVV LGKDLVPRIG LSQLEGFRRQ
   541  LLDVLQRSTK PKWRIIVGAT KCIPKSELPE EVEVTTLAST RLWTHPSDLT IALSASTPLY
   601  PPGRIIHVVH NHPAEQCCCC EQEEPTYFAI WGDNKAFNEV IISPAMLHEH LPYVVMEGLN
   661  KVLENYNKGK TALLSAAKVM VSPTEVDLTP ELIFQQQPLP TGPPMPTGLA LELPTADHRN
   721  SSVRSKSQSE MSLEGFSEGR LLSPVVAAAA RQDPVELLLL STQERLAAEL QARRAPLATM
   781  ESLSDTESLY SFDSRRSSGF RSIRGSPSLH AVLERDEGHL FYIDPAIPEE NPSLSSRTEL
   841  LAADSLSKHS QDTQPLEAAL GSGGVTPERP PSAAANDEEE EVGGGGGGPA SRGELALHNG
   901  RLGDSPSPQV LEFAEFIDSL FNLDSKSSSF QDLYCMVVPE SPTSDYAEGP KSPSQQEILL
   961  RAQFEPNLVP KPPRLFAGSA DPSSGISLSP SFPLSSSGEL MDLTPTGLSS QECLAADKIR
  1021  TSTPTGHGAS PAKQDELVIS AR

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against DAGLA can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Cell surface

Secreted

No

Transmembrane segments

4

Mean surface accessibility (rSASA)

0.42

Highest tissue expression

12 nTPM

Expression across tissuesHPA

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about DAGLA.

Disease | AllUniProt

Conditions DAGLA is implicated in, by any mechanism.

• Spinocerebellar ataxia 20 (SCA20) MIM:608687
• Neuroocular syndrome 2, paroxysmal type (NOC2) MIM:168885

Disease | GeneticClinVar

7 pathogenic / likely-pathogenic of 177 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

• Benign paroxysmal tonic upgaze of childhood with ataxia
• See cases
• Inborn genetic diseases

ReferencesPubMed · IEDB

Publications for DAGLA from three distinct lines of evidence, kept separate because they answer different questions: whether antibodies are directed at the protein, whether a B-cell epitope has been mapped on it, and whether a T-cell epitope has. Each is labelled with its source.

Sources: PubMed — antigen-level antibody evidence from a custom retrieval. Records matching a controlled set of autoantibody terms (the MeSH descriptors Autoantibodies and Autoantigens, with title and abstract term variants) were obtained through NCBI E-utilities, and their titles and abstracts parsed for constructions that direct an antibody at a named protein rather than for co-occurrence. Captured names were resolved against UniProt nomenclature and each antigen adjudicated individually against the source text. Bibliographic records from PubMed and MeSH, U.S. National Library of Medicine; citation metrics from NIH iCite (Hutchins et al., PLoS Biology 2016). Titles link to PubMed; abstracts are not reproduced here. The NLM does not endorse this analysis.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.17

gnomAD pLI

1

gnomAD missense Z

4.01

DepMap mean gene effect

-0.07

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• arachidonate metabolic process
• cannabinoid biosynthetic process
• diacylglycerol catabolic process
• monoacylglycerol biosynthetic process
• neuroblast proliferation
• regulation of neuroinflammatory response
• retrograde trans-synaptic signaling by endocannabinoid

Molecular functions

• lipoprotein lipase activity
• metal ion binding
• monoacylglycerol lipase activity

Cellular components

• cytoplasm
• dendrite membrane
• dendritic spine membrane
• early endosome membrane
• plasma membrane
• postsynaptic density membrane
• postsynaptic membrane
• varicosity

Protein domainsUniProt · Pfam · InterPro

• Fungal lipase-type domain
• Alpha/Beta hydrolase fold
• Diacylglycerol Lipase-Related
• Lipase (class 3)

KeywordsUniProt

• Calcium
• Cell membrane
• Cell projection
• Endosome
• Glycoprotein
• Hydrolase
• Intellectual disability
• Lipid degradation
• Lipid metabolism
• Membrane
• Metal-binding
• Neurodegeneration
• Phosphoprotein
• Postsynaptic cell membrane
• Spinocerebellar ataxia
• Synapse
• Transmembrane
• Transmembrane helix

InteractionsUniProt · HPA

Protein binding partners of DAGLA in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• CAMK2A
• HOMER2
• HOMER1