Seroatlas · Human Serome Atlas

CSTF3

Cleavage stimulation factor subunit 3

Also known as: CstF-77, CSTF3_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q12996
Gene: CSTF3
Ensembl: ENSG00000176102
Chromosome: 11
Canonical length: 717 aa
Protein class: Predicted intracellular proteins
Subcellular location: Nucleoplasm
Quaternary structure: Homodimer

OverviewNCBI Gene

The protein encoded by this gene is one of three (including CSTF1 and CSTF2) cleavage stimulation factors that combine to form the cleavage stimulation factor complex (CSTF). This complex is involved in the polyadenylation and 3' end cleavage of pre-mRNAs. The encoded protein functions as a homodimer and interacts directly with both CSTF1 and CSTF2 in the CSTF complex. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

717 residues, UniProt reviewed canonical sequence.

>Q12996|CSTF3
     1  MSGDGATEQA AEYVPEKVKK AEKKLEENPY DLDAWSILIR EAQNQPIDKA RKTYERLVAQ
    61  FPSSGRFWKL YIEAEIKAKN YDKVEKLFQR CLMKVLHIDL WKCYLSYVRE TKGKLPSYKE
   121  KMAQAYDFAL DKIGMEIMSY QIWVDYINFL KGVEAVGSYA ENQRITAVRR VYQRGCVNPM
   181  INIEQLWRDY NKYEEGINIH LAKKMIEDRS RDYMNARRVA KEYETVMKGL DRNAPSVPPQ
   241  NTPQEAQQVD MWKKYIQWEK SNPLRTEDQT LITKRVMFAY EQCLLVLGHH PDIWYEAAQY
   301  LEQSSKLLAE KGDMNNAKLF SDEAANIYER AISTLLKKNM LLYFAYADYE ESRMKYEKVH
   361  SIYNRLLAIE DIDPTLVYIQ YMKFARRAEG IKSGRMIFKK AREDTRTRHH VYVTAALMEY
   421  YCSKDKSVAF KIFELGLKKY GDIPEYVLAY IDYLSHLNED NNTRVLFERV LTSGSLPPEK
   481  SGEIWARFLA FESNIGDLAS ILKVEKRRFT AFKEEYEGKE TALLVDRYKF MDLYPCSASE
   541  LKALGYKDVS RAKLAAIIPD PVVAPSIVPV LKDEVDRKPE YPKPDTQQMI PFQPRHLAPP
   601  GLHPVPGGVF PVPPAAVVLM KLLPPPICFQ GPFVQVDELM EIFRRCKIPN TVEEAVRIIT
   661  GGAPELAVEG NGPVESNAVL TKAVKRPNED SDEDEEKGAV VPPVHDIYRA RQQKRIR

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against CSTF3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.35
Highest tissue expression: 58 nTPM

Expression across tissuesHPA

Tissue

epididymis: 58 nTPM
pituitary gland: 54 nTPM
cerebellum: 40 nTPM
hypothalamus: 33 nTPM
liver: 32 nTPM
testis: 31 nTPM

Single-cell type

epididymal principal cells: 245 nCPM
sertoli cells: 167 nCPM
corticotrophs: 133 nCPM
lactotrophs: 126 nCPM
gonadotrophs: 111 nCPM
neutrophil progenitors: 109 nCPM

Immune cell

T-reg: 9.1 nTPM
non-classical monocyte: 8.3 nTPM
naive CD8 T-cell: 7.9 nTPM
myeloid DC: 7.6 nTPM
naive CD4 T-cell: 7.5 nTPM
memory CD4 T-cell: 7.2 nTPM

Brain region

cerebellum: 53 nTPM
hypothalamus: 50 nTPM
white matter: 50 nTPM
cerebral cortex: 46 nTPM
choroid plexus: 46 nTPM
hippocampal formation: 44 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.3
gnomAD pLI: 0.98
gnomAD missense Z: 4.94
DepMap mean gene effect: -1.17
DepMap dependency class: common

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

HAT (Half-A-TPR) repeat
Suppressor of forked
Tetratricopeptide-like helical domain superfamily
Suppressor of forked protein (Suf)
mRNA 3'-end-processing protein Rna14-like

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of CSTF3 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads CSTF3 as an antibody target. Whether an autoantibody or antibody against CSTF3 could matter depends on whether native CSTF3 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

CSTF3 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label CSTF3 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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