Seroatlas · Human Serome Atlas

CRACD

Capping protein-inhibiting regulator of actin dynamics

Also known as: CRACD_HUMAN, CRAD, KIAA1211

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q6ZU35
Gene: CRACD
Ensembl: ENSG00000109265
Chromosome: 4
Canonical length: 1233 aa
Protein class: Disease related genes, Predicted intracellular proteins
Subcellular location: Nucleoplasm,Golgi apparatus

OverviewNCBI Gene

Involved in negative regulation of barbed-end actin filament capping. Predicted to be located in cytosol. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

1233 residues, UniProt reviewed canonical sequence.

>Q6ZU35|CRACD
     1  MGTRAFSHDS IFIPDGGAES EQTVQAMSQD NILGKVKTLQ QQLGKNIKFG QRSPNAIPMN
    61  KANSGEASLE EDLFLTSPME IVTQQDIVLS DAENKSSDTP SSLSPLNLPG AGSEMEEKVA
   121  PVKPSRPKRH FSSAGTIESV NLDAIPLAIA RLDNSAAKHK LAVKPKKQRV SKKHRRLAQD
   181  PQHEQGGLES RPCLDQNGHP GEDKPTWHEE EPNPLDSEEE RRRQEDYWRE LEAKCKRQKA
   241  EAAEKRRLEE QRLQALERRL WEENRRQELL EEEGEGQEPP LEAERAPREE QQRSLEAPGW
   301  EDAERREREE RERLEAEEER RRLQAQAQAE ERRRLEEDAR LEERRRQEEE EGRCAEELKR
   361  QEEEEAEGWE ELEQQEAEVQ GPPEALEETG EGRRGAEEED LGEEEEEGQA HLEDWRGQLS
   421  ELLNDFEERL EDQERLKPEG QREHSEEPGI CEEQNPEAER RREQQGRSGD FQGADRPGPE
   481  EKREEGDTEP LLKQEGPVEA AQPPVERKEA AALEQGRKVE ELRWQEVDER QTMPRPYTFQ
   541  VSSGGKQILF PKVNLSPVTP AKDTGLTAAP QEPKAPKASP VQHALPSSLS VPHTAILVTG
   601  AQLCGPAVNL SQIKDTACKS LLGLEEKKHA EAPAGENPPR GPGDARAGSG KAKPRQESPS
   661  SASALAEWAS IRSRILKNAE SDPRSSERDQ LRPGDESTPR GRCDSRGNQR KTPPVNAKFS
   721  IMPAWQKFSD GGTETSKQST EAESIRKRPM LGPSEETAPQ PPPAGVRELG KGPEKSEMHR
   781  EPADTTEGCK FAKDLPSFLV PSLPYPPQKV VAHTEFTTSS DSETANGIAK PDPVMPGGEE
   841  KASPFGIKLR RTNYSLRFNC DQQAEQKKKK RHSSTGDSAD AGPPAAGSAR GEKEMEGVAL
   901  KHGPSLPQER KQAPSTRRDS AEPSSSRSVP VAHPGPPPAS SQTPAPEHDK AANKMPLAQK
   961  PALAPKPTSQ TPPASPLSKL SRPYLVELLS RRAGRPDPEP SEPSKEDQES SDRRPPSPPG
  1021  PEERKGQKRD EEEEATERKP ASPPLPATQQ EKPSQTPEAG RKEKPMLQSR HSLDGSKLTE
  1081  KVETAQPLWI TLALQKQKGF REQQATREER KQAREAKQAE KLSKENVSVS VQPGSSSVSR
  1141  AGSLHKSTAL PEEKRPETAV SRLERREQLK KANTLPTSVT VEISDSAPPA PLVKEVTKRF
  1201  STPDAAPVST EPAWLALAKR KAKAWSDCPQ IIK

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against CRACD can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.65
Highest tissue expression: 27 nTPM

Expression across tissuesHPA

Tissue

testis: 27 nTPM
small intestine: 18 nTPM
duodenum: 13 nTPM
gallbladder: 11 nTPM
colon: 8.5 nTPM
rectum: 7.2 nTPM

Single-cell type

megakaryocyte-erythroid progenitors: 680 nCPM
podocytes: 462 nCPM
enterocytes: 432 nCPM
megakaryocyte progenitors: 422 nCPM
erythrocyte progenitors: 393 nCPM
retinal pigment epithelial cells: 367 nCPM

Immune cell

naive B-cell: 0.1 nTPM
basophil: 0 nTPM
classical monocyte: 0 nTPM
eosinophil: 0 nTPM
gdT-cell: 0 nTPM
intermediate monocyte: 0 nTPM

Brain region

pons: 43 nTPM
medulla oblongata: 39 nTPM
midbrain: 34 nTPM
hypothalamus: 30 nTPM
thalamus: 30 nTPM
basal ganglia: 26 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.53
gnomAD pLI: 0
DepMap mean gene effect: -0.14
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Cellular components

cytosol

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of CRACD in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads CRACD as an antibody target. Whether an autoantibody or antibody against CRACD could matter depends on whether native CRACD is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

CRACD is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label CRACD as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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