Seroatlas · Human Serome Atlas

CPSF7

Cleavage and polyadenylation specificity factor subunit 7

Also known as: CFIM59, CPSF7_HUMAN, FLJ12529

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q8N684
Gene
CPSF7
Ensembl
ENSG00000149532
Chromosome
11
Canonical length
471 aa
Protein class
Predicted intracellular proteins
Subcellular location
Nucleoplasm,Cytosol

OverviewNCBI Gene

Cleavage factor Im (CFIm) is one of six factors necessary for correct cleavage and polyadenylation of pre-mRNAs. CFIm is composed of three different subunits of 25, 59, and 68 kDa, and it functions as a heterotetramer, with a dimer of the 25 kDa subunit binding to two of the 59 or 68 kDa subunits. The protein encoded by this gene represents the 59 kDa subunit, which can interact with the splicing factor U2 snRNP Auxiliary Factor (U2AF) 65 to link the splicing and polyadenylation complexes. [provided by RefSeq, Oct 2016]

Canonical amino-acid sequenceUniProt

471 residues, UniProt reviewed canonical sequence.

>Q8N684|CPSF7
     1  MSEGVDLIDI YADEEFNQDP EFNNTDQIDL YDDVLTATSQ PSDDRSSSTE PPPPVRQEPS
    61  PKPNNKTPAI LYTYSGLRNR RAAVYVGSFS WWTTDQQLIQ VIRSIGVYDV VELKFAENRA
   121  NGQSKGYAEV VVASENSVHK LLELLPGKVL NGEKVDVRPA TRQNLSQFEA QARKRECVRV
   181  PRGGIPPRAH SRDSSDSADG RATPSENLVP SSARVDKPPS VLPYFNRPPS ALPLMGLPPP
   241  PIPPPPPLSS SFGVPPPPPG IHYQHLMPPP PRLPPHLAVP PPGAIPPALH LNPAFFPPPN
   301  ATVGPPPDTY MKASAPYNHH GSRDSGPPPS TVSEAEFEDI MKRNRAISSS AISKAVSGAS
   361  AGDYSDAIET LLTAIAVIKQ SRVANDERCR VLISSLKDCL HGIEAKSYSV GASGSSSRKR
   421  HRSRERSPSR SRESSRRHRD LLHNEDRHDD YFQERNREHE RHRDRERDRH H

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against CPSF7 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.57
Highest tissue expression
77 nTPM

Expression across tissuesHPA

Tissue

  • pituitary gland: 77 nTPM
  • skeletal muscle: 65 nTPM
  • liver: 62 nTPM
  • thyroid gland: 57 nTPM
  • blood vessel: 56 nTPM
  • spleen: 55 nTPM

Single-cell type

  • neutrophils: 183 nCPM
  • somatotrophs: 91 nCPM
  • lactotrophs: 90 nCPM
  • astrocytes: 90 nCPM
  • neutrophil progenitors: 89 nCPM
  • adrenal cortex cells: 88 nCPM

Immune cell

  • basophil: 15 nTPM
  • neutrophil: 9.9 nTPM
  • MAIT T-cell: 8.7 nTPM
  • memory CD8 T-cell: 8.4 nTPM
  • gdT-cell: 8.1 nTPM
  • myeloid DC: 7.6 nTPM

Brain region

  • cerebral cortex: 93 nTPM
  • white matter: 67 nTPM
  • midbrain: 64 nTPM
  • hypothalamus: 63 nTPM
  • cerebellum: 61 nTPM
  • choroid plexus: 60 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.2
gnomAD pLI
1
gnomAD missense Z
2.59
DepMap mean gene effect
-0.19
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of CPSF7 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads CPSF7 as an antibody target. Whether an autoantibody or antibody against CPSF7 could matter depends on whether native CPSF7 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

CPSF7 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label CPSF7 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/CPSF7. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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