Seroatlas · Human Serome Atlas

CNKSR3

Connector enhancer of kinase suppressor of ras 3

Also known as: CNKR3_HUMAN, FLJ31349, MAGI1

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q6P9H4
Gene
CNKSR3
Ensembl
ENSG00000153721
Chromosome
6
Canonical length
555 aa
Protein class
Predicted intracellular proteins
Subcellular location
Mitochondria,Cytosol

OverviewNCBI Gene

Predicted to be involved in negative regulation of ERK1 and ERK2 cascade; negative regulation of peptidyl-serine phosphorylation; and positive regulation of sodium ion transport. Predicted to act upstream of or within positive regulation of sodium ion transmembrane transporter activity. Predicted to be located in apical plasma membrane and cytoplasm. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

555 residues, UniProt reviewed canonical sequence.

>Q6P9H4|CNKSR3
     1  MEPVTKWSPK QVVDWTRGLD DCLQQYVHKF EREKINGEQL LQISHQDLEE LGVTRIGHQE
    61  LVLEAVDLLC ALNYGLETDN MKNLVLKLRA SSHNLQNYIS SRRKSPAYDG NTSRKAPNEF
   121  LTSVVELIGA AKALLAWLDR APFTGITDFS VTKNKIIQLC LDLTTTVQKD CFVAEMEDKV
   181  LTVVKVLNGI CDKTIRSTTD PVMSQCACLE EVHLPNIKPG EGLGMYIKST YDGLHVITGT
   241  TENSPADRSQ KIHAGDEVIQ VNQQTVVGWQ LKNLVKKLRE NPTGVVLLLK KRPTGSFNFT
   301  PAPLKNLRWK PPLVQTSPPP ATTQSPESTM DTSLKKEKSA ILDLYIPPPP AVPYSPRDEN
   361  GSFVYGGSSK CKQPLPGPKG SESPNSFLDQ ESRRRRFTIA DSDQLPGYSV ETNILPTKMR
   421  EKTPSYGKPR PLSMPADGNW MGIVDPFARP RGHGRKGEDA LCRYFSNERI PPIIEESSSP
   481  PYRFSRPTTE RHLVRGADYI RGSRCYINSD LHSSATIPFQ EEGTKKKSGS SATKSSSTEP
   541  SLLVSWFTRL KLLTH

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against CNKSR3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Cell surface
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.47
Highest tissue expression
18 nTPM

Expression across tissuesHPA

Tissue

  • pancreas: 18 nTPM
  • esophagus: 12 nTPM
  • liver: 9 nTPM
  • endometrium: 7.1 nTPM
  • kidney: 7 nTPM
  • choroid plexus: 6.5 nTPM

Single-cell type

  • proximal tubule cells: 559 nCPM
  • renal connecting tubule cells: 282 nCPM
  • distal convoluted tubule cells: 265 nCPM
  • podocytes: 262 nCPM
  • loop of henle epithelial cells: 259 nCPM
  • renal collecting duct intercalated cells: 151 nCPM

Immune cell

  • neutrophil: 0.6 nTPM
  • basophil: 0.5 nTPM
  • plasmacytoid DC: 0.2 nTPM
  • eosinophil: 0.1 nTPM
  • naive B-cell: 0.1 nTPM
  • NK-cell: 0.1 nTPM

Brain region

  • choroid plexus: 42 nTPM
  • thalamus: 37 nTPM
  • white matter: 36 nTPM
  • midbrain: 28 nTPM
  • medulla oblongata: 26 nTPM
  • basal ganglia: 25 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.49
gnomAD pLI
0.03
gnomAD missense Z
0.59
DepMap mean gene effect
0.15
DepMap dependency class
none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of CNKSR3 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads CNKSR3 as an antibody target. Whether an autoantibody or antibody against CNKSR3 could matter depends on whether native CNKSR3 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

CNKSR3 is annotated at the cell surface, where native CNKSR3 is exposed to circulating antibodies and is a prime autoantibody target that could block, deplete, or overstimulate it.

Annotation status

The present source text does not explicitly label CNKSR3 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/CNKSR3. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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