CKM

Protein identityUniProt · HPA

UniProt accession

P06732

Gene

CKM

Ensembl

ENSG00000104879

Chromosome

19

Canonical length

381 aa

Protein class

Candidate cardiovascular disease genes, Enzymes, FDA approved drug targets, Metabolic proteins, Plasma proteins, Predicted intracellular proteins

OverviewNCBI Gene

The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

381 residues, UniProt reviewed canonical sequence.

>P06732|CKM
     1  MPFGNTHNKF KLNYKPEEEY PDLSKHNNHM AKVLTLELYK KLRDKETPSG FTVDDVIQTG
    61  VDNPGHPFIM TVGCVAGDEE SYEVFKELFD PIISDRHGGY KPTDKHKTDL NHENLKGGDD
   121  LDPNYVLSSR VRTGRSIKGY TLPPHCSRGE RRAVEKLSVE ALNSLTGEFK GKYYPLKSMT
   181  EKEQQQLIDD HFLFDKPVSP LLLASGMARD WPDARGIWHN DNKSFLVWVN EEDHLRVISM
   241  EKGGNMKEVF RRFCVGLQKI EEIFKKAGHP FMWNQHLGYV LTCPSNLGTG LRGGVHVKLA
   301  HLSKHPKFEE ILTRLRLQKR GTGGVDTAAV GSVFDVSNAD RLGSSEVEQV QLVVDGVKLM
   361  VEMEKKLEKG QSIDDMIPAQ K

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against CKM can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.22

Highest tissue expression

68,909 nTPM

Expression across tissuesHPA

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about CKM.

Disease | ImmuneIEDB

Conditions an epitope on CKM was assayed in.

• multiple sclerosis B cell

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.79

gnomAD pLI

0.01

gnomAD missense Z

0.66

DepMap mean gene effect

0.05

DepMap dependency class

none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 2% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• phosphocreatine biosynthetic process

Molecular functions

• ATP binding
• creatine kinase activity

Cellular components

• cytosol
• extracellular space

Protein domainsUniProt · Pfam · InterPro

• ATP:guanido phosphotransferase
• Glutamine synthetase/guanido kinase, catalytic domain
• ATP:guanido phosphotransferase, N-terminal
• ATP:guanido phosphotransferase, catalytic domain
• ATP:guanido phosphotransferase active site
• ATP:guanido phosphotransferase, N-terminal domain superfamily
• ATP:guanido phosphotransferase, C-terminal catalytic domain
• ATP:guanido phosphotransferase, N-terminal domain

KeywordsUniProt

• ATP-binding
• Cytoplasm
• Kinase
• Nucleotide-binding
• Phosphoprotein
• Transferase

InteractionsUniProt · HPA

Protein binding partners of CKM in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• TRIM63
• ASB9
• CKB
• CKMT1A