CKB

Protein identityUniProt · HPA

UniProt accession

P12277

Gene

CKB

Ensembl

ENSG00000166165

Chromosome

14

Canonical length

381 aa

Protein class

Cancer-related genes, Candidate cardiovascular disease genes, Enzymes, FDA approved drug targets, Metabolic proteins, Plasma proteins, Predicted intracellular proteins

Subcellular location

Cytosol

OverviewNCBI Gene

The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. A pseudogene of this gene has been characterized. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

381 residues, UniProt reviewed canonical sequence.

>P12277|CKB
     1  MPFSNSHNAL KLRFPAEDEF PDLSAHNNHM AKVLTPELYA ELRAKSTPSG FTLDDVIQTG
    61  VDNPGHPYIM TVGCVAGDEE SYEVFKDLFD PIIEDRHGGY KPSDEHKTDL NPDNLQGGDD
   121  LDPNYVLSSR VRTGRSIRGF CLPPHCSRGE RRAIEKLAVE ALSSLDGDLA GRYYALKSMT
   181  EAEQQQLIDD HFLFDKPVSP LLLASGMARD WPDARGIWHN DNKTFLVWVN EEDHLRVISM
   241  QKGGNMKEVF TRFCTGLTQI ETLFKSKDYE FMWNPHLGYI LTCPSNLGTG LRAGVHIKLP
   301  NLGKHEKFSE VLKRLRLQKR GTGGVDTAAV GGVFDVSNAD RLGFSEVELV QMVVDGVKLL
   361  IEMEQRLEQG QAIDDLMPAQ K

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against CKB can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Cell surface

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.23

Highest tissue expression

1,487 nTPM

Expression across tissuesHPA

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about CKB.

Disease | ImmuneIEDB

Conditions an epitope on CKB was assayed in.

• multiple sclerosis B cell

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.58

gnomAD pLI

0.22

gnomAD missense Z

2.32

DepMap mean gene effect

0.05

DepMap dependency class

none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• futile creatine cycle
• phosphocreatine biosynthetic process
• substantia nigra development

Molecular functions

• ATP binding
• creatine kinase activity
• ubiquitin protein ligase binding

Cellular components

• cytosol
• extracellular exosome
• extracellular space
• mitochondrion
• plasma membrane

Protein domainsUniProt · Pfam · InterPro

• ATP:guanido phosphotransferase
• Glutamine synthetase/guanido kinase, catalytic domain
• ATP:guanido phosphotransferase, N-terminal
• ATP:guanido phosphotransferase, catalytic domain
• ATP:guanido phosphotransferase active site
• ATP:guanido phosphotransferase, N-terminal domain superfamily
• ATP:guanido phosphotransferase, C-terminal catalytic domain
• ATP:guanido phosphotransferase, N-terminal domain

KeywordsUniProt

• ATP-binding
• Cell membrane
• Cytoplasm
• Isopeptide bond
• Kinase
• Membrane
• Mitochondrion
• Nitration
• Nucleotide-binding
• Phosphoprotein
• Transferase
• Ubl conjugation

InteractionsUniProt · HPA

Protein binding partners of CKB in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• SLC12A6
• ASB9
• CKM
• CUL3