Seroatlas · Human Serome Atlas

CFL1

Cofilin-1

Also known as: CFL, COF1_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
P23528
Gene
CFL1
Ensembl
ENSG00000172757
Chromosome
11
Canonical length
166 aa
Protein class
Plasma proteins, Predicted intracellular proteins
Subcellular location
Plasma membrane

OverviewNCBI Gene

The protein encoded by this gene can polymerize and depolymerize F-actin and G-actin in a pH-dependent manner. Increased phosphorylation of this protein by LIM kinase aids in Rho-induced reorganization of the actin cytoskeleton. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.[supplied by OMIM, Apr 2004]

Canonical amino-acid sequenceUniProt

166 residues, UniProt reviewed canonical sequence.

>P23528|CFL1
     1  MASGVAVSDG VIKVFNDMKV RKSSTPEEVK KRKKAVLFCL SEDKKNIILE EGKEILVGDV
    61  GQTVDDPYAT FVKMLPDKDC RYALYDATYE TKESKKEDLV FIFWAPESAP LKSKMIYASS
   121  KDAIKKKLTG IKHELQANCY EEVKDRCTLA EKLGGSAVIS LEGKPL

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against CFL1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.29
Highest tissue expression
1,247 nTPM

Expression across tissuesHPA

Tissue

  • bone marrow: 1,247 nTPM
  • duodenum: 1,098 nTPM
  • colon: 1,071 nTPM
  • small intestine: 1,054 nTPM
  • tonsil: 1,029 nTPM
  • cerebral cortex: 968 nTPM

Single-cell type

  • hofbauer cells: 3,412 nCPM
  • extravillous trophoblasts: 3,158 nCPM
  • megakaryocytes: 2,825 nCPM
  • esophageal apical cells: 2,706 nCPM
  • colonocytes: 2,419 nCPM
  • decidual stromal cells: 2,071 nCPM

Immune cell

  • total PBMC: 6,854 nTPM
  • myeloid DC: 3,351 nTPM
  • non-classical monocyte: 3,274 nTPM
  • eosinophil: 2,984 nTPM
  • neutrophil: 2,965 nTPM
  • intermediate monocyte: 2,962 nTPM

Brain region

  • white matter: 804 nTPM
  • thalamus: 694 nTPM
  • hippocampal formation: 677 nTPM
  • medulla oblongata: 674 nTPM
  • cerebral cortex: 669 nTPM
  • pons: 643 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about CFL1.

Disease | ImmuneIEDB

Conditions an epitope on CFL1 was assayed in.

ReferencesPubMed · IEDB

Publications for CFL1 from three distinct lines of evidence, kept separate because they answer different questions: whether antibodies are directed at the protein, whether a B-cell epitope has been mapped on it, and whether a T-cell epitope has. Each is labelled with its source.

Reference: AutoantibodyPubMed

1 publication

Reference: T cellIEDB

1 publication

Sources: PubMed — antigen-level antibody evidence from a custom retrieval. Records matching a controlled set of autoantibody terms (the MeSH descriptors Autoantibodies and Autoantigens, with title and abstract term variants) were obtained through NCBI E-utilities, and their titles and abstracts parsed for constructions that direct an antibody at a named protein rather than for co-occurrence. Captured names were resolved against UniProt nomenclature and each antigen adjudicated individually against the source text. IEDB — curated epitope assays from the Immune Epitope Database (Vita et al., Nucleic Acids Research 2019). Bibliographic records from PubMed and MeSH, U.S. National Library of Medicine; citation metrics from NIH iCite (Hutchins et al., PLoS Biology 2016). Titles link to PubMed; abstracts are not reproduced here. The NLM does not endorse this analysis.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.72
gnomAD pLI
0.56
gnomAD missense Z
2.8
DepMap mean gene effect
-0.57
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of CFL1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads CFL1 as an antibody target. Whether an autoantibody or antibody against CFL1 could matter depends on whether native CFL1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

CFL1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label CFL1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/CFL1. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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