BTAF1

Protein identityUniProt · HPA

UniProt accession

O14981

Gene

BTAF1

Ensembl

ENSG00000095564

Chromosome

10

Canonical length

1849 aa

Protein class

Plasma proteins, Predicted intracellular proteins

Subcellular location

Nucleoplasm,Vesicles

OverviewNCBI Gene

This gene encodes a TAF (TATA box-binding protein-associated factor), which associates with TBP (TATA box-binding protein) to form the B-TFIID complex that is required for transcription initiation of genes by RNA polymerase II. This TAF has DNA-dependent ATPase activity, which drives the dissociation of TBP from DNA, freeing the TBP to associate with other TATA boxes or TATA-less promoters. [provided by RefSeq, Sep 2011]

Canonical amino-acid sequenceUniProt

1849 residues, UniProt reviewed canonical sequence.

>O14981|BTAF1
     1  MAVSRLDRLF ILLDTGTTPV TRKAAAQQLG EVVKLHPHEL NNLLSKVLIY LRSANWDTRI
    61  AAGQAVEAIV KNVPEWNPVP RTRQEPTSES SMEDSPTTER LNFDRFDICR LLQHGASLLG
   121  SAGAEFEVQD EKSGEVDPKE RIARQRKLLQ KKLGLNMGEA IGMSTEELFN DEDLDYTPTS
   181  ASFVNKQPTL QAAELIDSEF RAGMSNRQKN KAKRMAKLFA KQRSRDAVET NEKSNDSTDG
   241  EPEEKRRKIA NVVINQSAND SKVLIDNIPD SSSLIEETNE WPLESFCEEL CNDLFNPSWE
   301  VRHGAGTGLR EILKAHGKSG GKMGDSTLEE MIQQHQEWLE DLVIRLLCVF ALDRFGDFVS
   361  DEVVAPVRET CAQTLGVVLK HMNETGVHKT VDVLLKLLTQ EQWEVRHGGL LGIKYALAVR
   421  QDVINTLLPK VLTRIIEGLQ DLDDDVRAVA AASLVPVVES LVYLQTQKVP FIINTLWDAL
   481  LELDDLTAST NSIMTLLSSL LTYPQVQQCS IQQSLTVLVP RVWPFLHHTI SSVRRAALET
   541  LFTLLSTQDQ NSSSWLIPIL PDMLRHIFQF CVLESSQEIL DLIHKVWMEL LSKASVQYVV
   601  AAACPWMGAW LCLMMQPSHL PIDLNMLLEV KARAKEKTGG KVRQGQSQNK EVLQEYIAGA
   661  DTIMEDPATR DFVVMRARMM AAKLLGALCC CICDPGVNVV TQEIKPAESL GQLLLFHLNS
   721  KSALQRISVA LVICEWAALQ KECKAVTLAV QPRLLDILSE HLYYDEIAVP FTRMQNECKQ
   781  LISSLADVHI EVGNRVNNNV LTIDQASDLV TTVFNEATSS FDLNPQVLQQ LDSKRQQVQM
   841  TVTETNQEWQ VLQLRVHTFA ACAVVSLQQL PEKLNPIIKP LMETIKKEEN TLVQNYAAQC
   901  IAKLLQQCTT RTPCPNSKII KNLCSSLCVD PYLTPCVTCP VPTQSGQENS KGSTSEKDGM
   961  HHTVTKHRGI ITLYRHQKAA FAITSRRGPT PKAVKAQIAD LPAGSSGNIL VELDEAQKPY
  1021  LVQRRGAEFA LTTIVKHFGG EMAVKLPHLW DAMVGPLRNT IDINNFDGKS LLDKGDSPAQ
  1081  ELVNSLQVFE TAAASMDSEL HPLLVQHLPH LYMCLQYPST AVRHMAARCV GVMSKIATME
  1141  TMNIFLEKVL PWLGAIDDSV KQEGAIEALA CVMEQLDVGI VPYIVLLVVP VLGRMSDQTD
  1201  SVRFMATQCF ATLIRLMPLE AGIPDPPNMS AELIQLKAKE RHFLEQLLDG KKLENYKIPV
  1261  PINAELRKYQ QDGVNWLAFL NKYKLHGILC DDMGLGKTLQ SICILAGDHC HRAQEYARSK
  1321  LAECMPLPSL VVCPPTLTGH WVDEVGKFCS REYLNPLHYT GPPTERIRLQ HQVKRHNLIV
  1381  ASYDVVRNDI DFFRNIKFNY CILDEGHVIK NGKTKLSKAV KQLTANYRII LSGTPIQNNV
  1441  LELWSLFDFL MPGFLGTERQ FAARYGKPIL ASRDARSSSR EQEAGVLAMD ALHRQVLPFL
  1501  LRRMKEDVLQ DLPPKIIQDY YCTLSPLQVQ LYEDFAKSRA KCDVDETVSS ATLSEETEKP
  1561  KLKATGHVFQ ALQYLRKLCN HPALVLTPQH PEFKTTAEKL AVQNSSLHDI QHAPKLSALK
  1621  QLLLDCGLGN GSTSESGTES VVAQHRILIF CQLKSMLDIV EHDLLKPHLP SVTYLRLDGS
  1681  IPPGQRHSIV SRFNNDPSID VLLLTTHVGG LGLNLTGADT VVFVEHDWNP MRDLQAMDRA
  1741  HRIGQKRVVN VYRLITRGTL EEKIMGLQKF KMNIANTVIS QENSSLQSMG TDQLLDLFTL
  1801  DKDGKAEKAD TSTSGKASMK SILENLSDLW DQEQYDSEYS LENFMHSLK

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against BTAF1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.29

Highest tissue expression

40 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.13

gnomAD pLI

1

gnomAD missense Z

4.38

DepMap mean gene effect

-0.43

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• negative regulation of chromatin binding
• negative regulation of DNA-templated transcription

Molecular functions

• ATP binding
• ATP hydrolysis activity
• ATP-dependent activity, acting on DNA
• DNA binding
• helicase activity
• TBP-class protein binding
• transcription coregulator activity

Cellular components

• nucleoplasm

Protein domainsUniProt · Pfam · InterPro

• SNF2, N-terminal domain
• Helicase, C-terminal domain-like
• Armadillo-like helical
• Helicase superfamily 1/2, ATP-binding domain
• Armadillo-type fold
• P-loop containing nucleoside triphosphate hydrolase
• SNF2-like, N-terminal domain superfamily
• SNF2/RAD5-like, C-terminal helicase domain
• SNF2-related domain
• Helicase conserved C-terminal domain
• Mot1, central domain
• Mot1, ATP-binding domain
• TATA-binding protein-associated factor Mot1
• Domain of unknown function (DUF3535)

KeywordsUniProt

• ATP-binding
• DNA-binding
• Helicase
• Hydrolase
• Nucleotide-binding
• Nucleus
• Phosphoprotein
• Repeat

InteractionsUniProt · HPA

Protein binding partners of BTAF1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• TBP