Seroatlas · Human Serome Atlas

B4GALT4

Beta-1,4-galactosyltransferase 4

Also known as: B4GT4_HUMAN, beta4Gal-T4

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: O60513
Gene: B4GALT4
Ensembl: ENSG00000121578
Chromosome: 3
Canonical length: 344 aa
Protein class: Enzymes, Metabolic proteins, Predicted intracellular proteins
Subcellular location: Golgi apparatus
Secretome location: Intracellular and membrane

OverviewNCBI Gene

This gene is one of seven beta-1,4-galactosyltransferase (beta4GalT) genes. They encode type II membrane-bound glycoproteins that appear to have exclusive specificity for the donor substrate UDP-galactose; all transfer galactose in a beta1,4 linkage to similar acceptor sugars: GlcNAc, Glc, and Xyl. Each beta4GalT has a distinct function in the biosynthesis of different glycoconjugates and saccharide structures. As type II membrane proteins, they have an N-terminal hydrophobic signal sequence that directs the protein to the Golgi apparatus and which then remains uncleaved to function as a transmembrane anchor. By sequence similarity, the beta4GalTs form four groups: beta4GalT1 and beta4GalT2, beta4GalT3 and beta4GalT4, beta4GalT5 and beta4GalT6, and beta4GalT7. The enzyme encoded by this gene appears to mainly play a role in glycolipid biosynthesis. Two alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

344 residues, UniProt reviewed canonical sequence.

>O60513|B4GALT4
     1  MGFNLTFHLS YKFRLLLLLT LCLTVVGWAT SNYFVGAIQE IPKAKEFMAN FHKTLILGKG
    61  KTLTNEASTK KVELDNCPSV SPYLRGQSKL IFKPDLTLEE VQAENPKVSR GRYRPQECKA
   121  LQRVAILVPH RNREKHLMYL LEHLHPFLQR QQLDYGIYVI HQAEGKKFNR AKLLNVGYLE
   181  ALKEENWDCF IFHDVDLVPE NDFNLYKCEE HPKHLVVGRN STGYRLRYSG YFGGVTALSR
   241  EQFFKVNGFS NNYWGWGGED DDLRLRVELQ RMKISRPLPE VGKYTMVFHT RDKGNEVNAE
   301  RMKLLHQVSR VWRTDGLSSC SYKLVSVEHN PLYINITVDF WFGA

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against B4GALT4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Secreted
Secreted: Yes
Transmembrane segments: 1
Mean surface accessibility (rSASA): 0.3
Highest tissue expression: 72 nTPM

Expression across tissuesHPA

Tissue

epididymis: 72 nTPM
urinary bladder: 35 nTPM
rectum: 33 nTPM
gallbladder: 31 nTPM
small intestine: 31 nTPM
colon: 29 nTPM

Single-cell type

extravillous trophoblasts: 189 nCPM
urothelial cells: 138 nCPM
epididymal principal cells: 127 nCPM
goblet cells: 117 nCPM
endometrial luminal cells: 108 nCPM
esophageal apical cells: 108 nCPM

Immune cell

basophil: 20 nTPM
NK-cell: 15 nTPM
naive CD4 T-cell: 14 nTPM
gdT-cell: 13 nTPM
eosinophil: 8.4 nTPM
memory CD8 T-cell: 8 nTPM

Brain region

choroid plexus: 16 nTPM
basal ganglia: 15 nTPM
hypothalamus: 13 nTPM
cerebral cortex: 13 nTPM
spinal cord: 13 nTPM
white matter: 13 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.14
gnomAD pLI: 0
gnomAD missense Z: 1.19
DepMap mean gene effect: 0.14
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of B4GALT4 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

SLC35A2

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads B4GALT4 as an antibody target. Whether an autoantibody or antibody against B4GALT4 could matter depends on whether native B4GALT4 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

B4GALT4 is annotated as secreted, so native B4GALT4 circulates and is directly accessible to antibodies. Secreted and cell-surface proteins are the autoantibody targets most likely to act like drugs, blocking or depleting the native protein.

Annotation status

The present source text does not explicitly label B4GALT4 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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