Seroatlas · Human Serome Atlas

ASTE1

Single-strand DNA endonuclease ASTE1

Also known as: ASTE1_HUMAN, HT001

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q2TB18
Gene: ASTE1
Ensembl: ENSG00000034533
Chromosome: 3
Canonical length: 679 aa
Protein class: Predicted intracellular proteins
Subcellular location: Endoplasmic reticulum

OverviewNCBI Gene

Enables 3' overhang single-stranded DNA endodeoxyribonuclease activity. Involved in double-strand break repair via homologous recombination and double-strand break repair via nonhomologous end joining. Implicated in Lynch syndrome and mismatch repair cancer syndrome. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

679 residues, UniProt reviewed canonical sequence.

>Q2TB18|ASTE1
     1  MGIRGLMSFV EDHSNEFFTD LKLRDTKIVI DGYALFHRLC FSSNLDLRYG GDYDSFADVV
    61  QKFFESLFAC NICPYVVLDG GCDISDKKLT TLKDRAREKI QMAHSLSVGG SGYVCPLLIR
   121  EVFIQVLIKL RVCFVQCFSE ADRDIMTLAN HWNCPVLSSD SDFCIFDLKT GFCPLNSFQW
   181  RNMNTIKGTQ NYIPAKCFSL DAFCHHFSNM NKALLPLFAV LCGNDHVNLP IMETFLSKAR
   241  LPLGATSSKG RRHHRILGLL NWLSHFANPT EALDNVLKYL PKKDRENVKE LLCCSMEEYQ
   301  QSQVKLQDFF QCGTYVCPDA LNLGLPEWVL VALAKGQLSP FISDALVLRR TILPTQVENM
   361  QQPNAHRISQ PIRQIIYGLL LNASPHLDKT SWNALPPQPL AFSEVERINK NIRTSIIDAV
   421  ELAKDHSDLS RLTELSLRRR QMLLLETLKV KQTILEPIPT SLKLPIAVSC YWLQHTETKA
   481  KLHHLQSLLL TMLVGPLIAI INSPGKEELQ EDGAKMLYAE FQRVKAQTRL GTRLDLDTAH
   541  IFCQWQSCLQ MGMYLNQLLS TPLPEPDLTR LYSGSLVHGL CQQLLASTSV ESLLSICPEA
   601  KQLYEYLFNA TRSYAPAEIF LPKGRSNSKK KRQKKQNTSC SKNRGRTTAH TKCWYEGNNR
   661  FGLLMVENLE EHSEASNIE

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ASTE1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Unknown
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.27
Highest tissue expression: 6.7 nTPM

Expression across tissuesHPA

Tissue

small intestine: 6.7 nTPM
thymus: 6.7 nTPM
thyroid gland: 6.6 nTPM
lymph node: 6.5 nTPM
tonsil: 6.4 nTPM
bone marrow: 6.3 nTPM

Single-cell type

epicardial cells: 66 nCPM
cardiomyocytes: 54 nCPM
late primary spermatocytes: 43 nCPM
tuft cells: 35 nCPM
early spermatids: 33 nCPM
innate lymphoid cells: 26 nCPM

Immune cell

basophil: 52 nTPM
NK-cell: 30 nTPM
eosinophil: 28 nTPM
naive CD4 T-cell: 28 nTPM
T-reg: 28 nTPM
memory CD4 T-cell: 22 nTPM

Brain region

cerebellum: 15 nTPM
cerebral cortex: 12 nTPM
white matter: 11 nTPM
choroid plexus: 8.9 nTPM
thalamus: 8.6 nTPM
hippocampal formation: 8.4 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.14
gnomAD pLI: 0
gnomAD missense Z: 0.86
DepMap mean gene effect: -0.01
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 10% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Hydrolase

InteractionsUniProt · HPA

Protein binding partners of ASTE1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads ASTE1 as an antibody target. Whether an autoantibody or antibody against ASTE1 could matter depends on whether native ASTE1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

ASTE1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label ASTE1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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