Seroatlas · Human Serome Atlas

ARHGAP4

Rho GTPase-activating protein 4

Also known as: C1, KIAA0131, p115, RHG04_HUMAN, RhoGAP4, SrGAP4

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: P98171
Gene: ARHGAP4
Ensembl: ENSG00000089820
Chromosome: X
Canonical length: 946 aa
Protein class: Predicted intracellular proteins
Subcellular location: Cytosol

OverviewNCBI Gene

This gene encodes a member of the rhoGAP family of proteins which play a role in the regulation of small GTP-binding proteins belonging to the RAS superfamily. The protein encoded by the orthologous gene in rat is localized to the Golgi complex and can redistribute to microtubules. The rat protein stimulates the activity of some Rho GTPases in vitro. Genomic deletions of this gene and a neighboring gene have been found in patients with nephrogenic diabetes insipidus. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]

Canonical amino-acid sequenceUniProt

946 residues, UniProt reviewed canonical sequence.

>P98171|ARHGAP4
     1  MAAHGKLRRE RGLQAEYETQ VKEMRWQLSE QLRCLELQGE LRRELLQELA EFMRRRAEVE
    61  LEYSRGLEKL AERFSSRGGR LGSSREHQSF RKEPSLLSPL HCWAVLLQHT RQQSRESAAL
   121  SEVLAGPLAQ RLSHIAEDVG RLVKKSRDLE QQLQDELLEV VSELQTAKKT YQAYHMESVN
   181  AEAKLREAER QEEKRAGRSV PTTTAGATEA GPLRKSSLKK GGRLVEKRQA KFMEHKLKCT
   241  KARNEYLLSL ASVNAAVSNY YLHDVLDLMD CCDTGFHLAL GQVLRSYTAA ESRTQASQVQ
   301  GLGSLEEAVE ALDPPGDKAK VLEVHATVFC PPLRFDYHPH DGDEVAEICV EMELRDEILP
   361  RAQNIQSRLD RQTIETEEVN KTLKATLQAL LEVVASDDGD VLDSFQTSPS TESLKSTSSD
   421  PGSRQAGRRR GQQQETETFY LTKLQEYLSG RSILAKLQAK HEKLQEALQR GDKEEQEVSW
   481  TQYTQRKFQK SRQPRPSSQY NQRLFGGDME KFIQSSGQPV PLVVESCIRF INLNGLQHEG
   541  IFRVSGAQLR VSEIRDAFER GEDPLVEGCT AHDLDSVAGV LKLYFRSLEP PLFPPDLFGE
   601  LLASSELEAT AERVEHVSRL LWRLPAPVLV VLRYLFTFLN HLAQYSDENM MDPYNLAVCF
   661  GPTLLPVPAG QDPVALQGRV NQLVQTLIVQ PDRVFPPLTS LPGPVYEKCM APPSASCLGD
   721  AQLESLGADN EPELEAEMPA QEDDLEGVVE AVACFAYTGR TAQELSFRRG DVLRLHERAS
   781  SDWWRGEHNG MRGLIPHKYI TLPAGTEKQV VGAGLQTAGE SGSSPEGLLA SELVHRPEPC
   841  TSPEAMGPSG HRRRCLVPAS PEQHVEVDKA VAQNMDSVFK ELLGKTSVRQ GLGPASTTSP
   901  SPGPRSPKAP PSSRLGRNKG FSRGPGAPAS PSASHPQGLD TTPKPH

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ARHGAP4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.41
Highest tissue expression: 114 nTPM

Expression across tissuesHPA

Tissue

spleen: 114 nTPM
bone marrow: 107 nTPM
lymph node: 65 nTPM
small intestine: 46 nTPM
appendix: 46 nTPM
thymus: 37 nTPM

Single-cell type

tuft cells: 92 nCPM
neutrophils: 61 nCPM
neutrophil progenitors: 54 nCPM
thymocytes: 43 nCPM
b-cells: 39 nCPM
pdcs: 36 nCPM

Immune cell

non-classical monocyte: 59 nTPM
intermediate monocyte: 42 nTPM
eosinophil: 35 nTPM
plasmacytoid DC: 31 nTPM
gdT-cell: 26 nTPM
classical monocyte: 26 nTPM

Brain region

choroid plexus: 28 nTPM
medulla oblongata: 18 nTPM
white matter: 17 nTPM
pons: 16 nTPM
thalamus: 15 nTPM
midbrain: 14 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.31
gnomAD pLI: 0.98
gnomAD missense Z: 0.69
DepMap mean gene effect: 0.09
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of ARHGAP4 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

NCKAP1L

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads ARHGAP4 as an antibody target. Whether an autoantibody or antibody against ARHGAP4 could matter depends on whether native ARHGAP4 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

ARHGAP4 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label ARHGAP4 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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