Seroatlas · Human Serome Atlas

NCKAP1L

Nck-associated protein 1-like

Also known as: HEM1, NCKPL_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
P55160
Gene
NCKAP1L
Ensembl
ENSG00000123338
Chromosome
12
Canonical length
1127 aa
Protein class
Disease related genes, Human disease related genes, Plasma proteins, Predicted membrane proteins
Subcellular location
Plasma membrane,Cytosol

OverviewNCBI Gene

This gene encodes a member of the HEM family of tissue-specific transmembrane proteins which are highly conserved from invertebrates through mammals. This gene is only expressed in hematopoietic cells. The encoded protein is a part of the Scar/WAVE complex which plays an important role in regulating cell shape in both metazoans and plants. Alternatively spliced transcript variants encoding different isoforms have been found.[provided by RefSeq, May 2010]

Canonical amino-acid sequenceUniProt

1127 residues, UniProt reviewed canonical sequence.

>P55160|NCKAP1L
     1  MSLTSAYQHK LAEKLTILND RGQGVLIRMY NIKKTCSDPK SKPPFLLEKS MEPSLKYINK
    61  KFPNIDVRNS TQHLGPVHRE KAEIIRFLTN YYQSFVDVME FRDHVYELLN TIDACQCHFD
   121  INLNFDFTRS YLDLIVTYTS VILLLSRIED RRILIGMYNC AHEMLHGHGD PSFARLGQMV
   181  LEYDHPLKKL TEEFGPHTKA VSGALLSLHF LFVRRNQGAE QWRSAQLLSL ISNPPAMINP
   241  ANSDTMACEY LSVEVMERWI IIGFLLCHGC LNSNSQCQKL WKLCLQGSLY ITLIREDVLQ
   301  VHKVTEDLFS SLKGYGKRVA DIKESKEHVI ANSGQFHCQR RQFLRMAVKE LETVLADEPG
   361  LLGPKALFAF MALSFIRDEV TWLVRHTENV TKTKTPEDYA DSSIAELLFL LEGIRSLVRR
   421  HIKVIQQYHL QYLARFDALV LSDIIQNLSV CPEEESIIMS SFVSILSSLN LKQVDNGEKF
   481  EFSGLRLDWF RLQAYTSVAK APLHLHENPD LAKVMNLIVF HSRMLDSVEK LLVETSDLST
   541  FCFHLRIFEK MFAMTLEESA MLRYAIAFPL ICAHFVHCTH EMCPEEYPHL KNHGLHHCNS
   601  FLEELAKQTS NCVLEICAEQ RNLSEQLLPK HCATTISKAK NKKTRKQRQT PRKGEPERDK
   661  PGAESHRKNR SIVTNMDKLH LNLTELALTM NHVYSFSVFE HTIFPSEYLS SHLEARLNRA
   721  IVWLAGYNAT TQEIVRPSEL LAGVKAYIGF IQSLAQFLGA DASRVIRNAL LQQTQPLDSC
   781  GEQTITTLYT NWYLESLLRQ ASSGTIILSP AMQAFVSLPR EGEQNFSAEE FSDISEMRAL
   841  AELLGPYGMK FLSENLMWHV TSQIVELKKL VVENMDILVQ IRSNFSKPDL MASLLPQLTG
   901  AENVLKRMTI IGVILSFRAM AQEGLREVFS SHCPFLMGPI ECLKEFVTPD TDIKVTLSIF
   961  ELASAAGVGC DIDPALVAAI ANLKADTSSP EEEYKVACLL LIFLAVSLPL LATDPSSFYS
  1021  IEKDGYNNNI HCLTKAIIQV SAALFTLYNK NIETHLKEFL VVASVSLLQL GQETDKLKTR
  1081  NRESISLLMR LVVEESSFLT LDMLESCFPY VLLRNAYREV SRAFHLN

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against NCKAP1L can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Cell surface
Secreted
No
Transmembrane segments
1
Mean surface accessibility (rSASA)
0.23
Highest tissue expression
43 nTPM

Expression across tissuesHPA

Tissue

  • spleen: 43 nTPM
  • tonsil: 41 nTPM
  • lymph node: 40 nTPM
  • thymus: 35 nTPM
  • appendix: 33 nTPM
  • bone marrow: 27 nTPM

Single-cell type

  • neutrophil progenitors: 263 nCPM
  • microglia: 263 nCPM
  • kupffer cells: 260 nCPM
  • neutrophils: 228 nCPM
  • monocytes: 227 nCPM
  • hofbauer cells: 200 nCPM

Immune cell

  • non-classical monocyte: 122 nTPM
  • intermediate monocyte: 104 nTPM
  • myeloid DC: 72 nTPM
  • classical monocyte: 69 nTPM
  • total PBMC: 65 nTPM
  • eosinophil: 55 nTPM

Brain region

  • white matter: 16 nTPM
  • thalamus: 13 nTPM
  • medulla oblongata: 13 nTPM
  • spinal cord: 9.7 nTPM
  • pons: 9.6 nTPM
  • midbrain: 7.7 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about NCKAP1L.

Disease | AllUniProt

Conditions NCKAP1L is implicated in, by any mechanism.

  • Immunodeficiency 72 with autoinflammation and lymphoproliferation (IMD72) MIM:618982

Disease | GeneticClinVar

12 pathogenic / likely-pathogenic of 576 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

  • Immunodeficiency 72 with autoinflammation

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.33
gnomAD pLI
0.85
gnomAD missense Z
2.48
DepMap mean gene effect
-0.16
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of NCKAP1L in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads NCKAP1L as an antibody target. Whether an autoantibody or antibody against NCKAP1L could matter depends on whether native NCKAP1L is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

NCKAP1L is annotated at the cell surface, where native NCKAP1L is exposed to circulating antibodies and is a prime autoantibody target that could block, deplete, or overstimulate it.

Annotation status

The present source text does not explicitly label NCKAP1L as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/NCKAP1L. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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