Seroatlas · Human Serome Atlas

ARFGAP1

ADP-ribosylation factor GTPase-activating protein 1

Also known as: ARF1GAP, ARFG1_HUMAN, bA261N11.3, FLJ10767

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q8N6T3
Gene
ARFGAP1
Ensembl
ENSG00000101199
Chromosome
20
Canonical length
406 aa
Protein class
Plasma proteins, Predicted intracellular proteins
Subcellular location
Nuclear membrane,Endoplasmic reticulum,Golgi apparatus,Vesicles,Primary cilium,Cytosol

OverviewNCBI Gene

The protein encoded by this gene is a GTPase-activating protein, which associates with the Golgi apparatus and which interacts with ADP-ribosylation factor 1. The encoded protein promotes hydrolysis of ADP-ribosylation factor 1-bound GTP and is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is required for the fusion of these vesicles with target compartments. The activity of this protein is stimulated by phosphoinosides and inhibited by phosphatidylcholine. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Canonical amino-acid sequenceUniProt

406 residues, UniProt reviewed canonical sequence.

>Q8N6T3|ARFGAP1
     1  MASPRTRKVL KEVRVQDENN VCFECGAFNP QWVSVTYGIW ICLECSGRHR GLGVHLSFVR
    61  SVTMDKWKDI ELEKMKAGGN AKFREFLESQ EDYDPCWSLQ EKYNSRAAAL FRDKVVALAE
   121  GREWSLESSP AQNWTPPQPR TLPSMVHRVS GQPQSVTASS DKAFEDWLND DLGSYQGAQG
   181  NRYVGFGNTP PPQKKEDDFL NNAMSSLYSG WSSFTTGASR FASAAKEGAT KFGSQASQKA
   241  SELGHSLNEN VLKPAQEKVK EGKIFDDVSS GVSQLASKVQ GVGSKGWRDV TTFFSGKAEG
   301  PLDSPSEGHS YQNSGLDHFQ NSNIDQSFWE TFGSAEPTKT RKSPSSDSWT CADTSTERRS
   361  SDSWEVWGSA STNRNSNSDG GEGGEGTKKA VPPAVPTDDG WDNQNW

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ARFGAP1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.57
Highest tissue expression
61 nTPM

Expression across tissuesHPA

Tissue

  • pituitary gland: 61 nTPM
  • cerebellum: 43 nTPM
  • cerebral cortex: 30 nTPM
  • pancreas: 27 nTPM
  • testis: 23 nTPM
  • basal ganglia: 20 nTPM

Single-cell type

  • somatotrophs: 91 nCPM
  • lactotrophs: 60 nCPM
  • proximal tubule cells: 55 nCPM
  • foveolar cells: 52 nCPM
  • astrocytes: 51 nCPM
  • goblet cells: 40 nCPM

Immune cell

  • non-classical monocyte: 9.6 nTPM
  • intermediate monocyte: 7.1 nTPM
  • eosinophil: 6.4 nTPM
  • MAIT T-cell: 6.4 nTPM
  • total PBMC: 6 nTPM
  • classical monocyte: 5.7 nTPM

Brain region

  • cerebral cortex: 50 nTPM
  • white matter: 42 nTPM
  • hippocampal formation: 40 nTPM
  • medulla oblongata: 40 nTPM
  • pons: 40 nTPM
  • amygdala: 38 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.6
gnomAD pLI
0
gnomAD missense Z
0.42
DepMap mean gene effect
0.03
DepMap dependency class
none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 12% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of ARFGAP1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads ARFGAP1 as an antibody target. Whether an autoantibody or antibody against ARFGAP1 could matter depends on whether native ARFGAP1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

ARFGAP1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label ARFGAP1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/ARFGAP1. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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