Seroatlas · Human Serome Atlas

AEN

Apoptosis-enhancing nuclease

Also known as: AEN_HUMAN, FLJ12484, FLJ12562, ISG20L1

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q8WTP8
Gene: AEN
Ensembl: ENSG00000181026
Chromosome: 15
Canonical length: 325 aa
Protein class: Predicted intracellular proteins
Subcellular location: Nucleoplasm,Nuclear membrane,Nucleoli

OverviewNCBI Gene

Enables DNA exonuclease activity. Involved in intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator and response to ionizing radiation. Located in nuclear membrane; nucleolus; and nucleoplasm. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

325 residues, UniProt reviewed canonical sequence.

>Q8WTP8|AEN
     1  MVPREAPESA QCLCPSLTIP NAKDVLRKRH KRRSRQHQRF MARKALLQEQ GLLSMPPEPG
    61  SSPLPTPFGA ATATEAASSG KQCLRAGSGS APCSRRPAPG KASGPLPSKC VAIDCEMVGT
   121  GPRGRVSELA RCSIVSYHGN VLYDKYIRPE MPIADYRTRW SGITRQHMRK AVPFQVAQKE
   181  ILKLLKGKVV VGHALHNDFQ ALKYVHPRSQ TRDTTYVPNF LSEPGLHTRA RVSLKDLALQ
   241  LLHKKIQVGQ HGHSSVEDAT TAMELYRLVE VQWEQQEARS LWTCPEDREP DSSTDMEQYM
   301  EDQYWPDDLA HGSRGGAREA QDRRN

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against AEN can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.47
Highest tissue expression: 22 nTPM

Expression across tissuesHPA

Tissue

pituitary gland: 22 nTPM
pancreas: 20 nTPM
bone marrow: 17 nTPM
spleen: 15 nTPM
skeletal muscle: 14 nTPM
liver: 13 nTPM

Single-cell type

late spermatids: 96 nCPM
early spermatids: 27 nCPM
breast hormone-responsive cells: 27 nCPM
breast secretory cells: 27 nCPM
ocular epithelial cells: 27 nCPM
endometrial ciliated cells: 26 nCPM

Immune cell

T-reg: 20 nTPM
MAIT T-cell: 12 nTPM
naive CD4 T-cell: 12 nTPM
memory B-cell: 11 nTPM
memory CD4 T-cell: 11 nTPM
naive CD8 T-cell: 11 nTPM

Brain region

hypothalamus: 16 nTPM
medulla oblongata: 10 nTPM
spinal cord: 8.9 nTPM
midbrain: 8.2 nTPM
pons: 8.1 nTPM
white matter: 8.1 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.88
gnomAD pLI: 0
gnomAD missense Z: 0
DepMap mean gene effect: 0.04
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of AEN in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

AAMP

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads AEN as an antibody target. Whether an autoantibody or antibody against AEN could matter depends on whether native AEN is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

AEN is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label AEN as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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