ACSS3

Protein identityUniProt · HPA

UniProt accession

Q9H6R3

Gene

ACSS3

Ensembl

ENSG00000111058

Chromosome

12

Canonical length

686 aa

Protein class

Enzymes, Metabolic proteins, Predicted membrane proteins

Subcellular location

Mitochondria

OverviewNCBI Gene

Enables propionate-CoA ligase activity. Predicted to be involved in ketone body biosynthetic process. Located in mitochondrial matrix. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

686 residues, UniProt reviewed canonical sequence.

>Q9H6R3|ACSS3
     1  MKPSWLQCRK VTSAGGLGGP LPGSSPARGA GAALRALVVP GPRGGLGGRG CRALSSGSGS
    61  EYKTHFAASV TDPERFWGKA AEQISWYKPW TKTLENKHSP STRWFVEGML NICYNAVDRH
   121  IENGKGDKIA IIYDSPVTNT KATFTYKEVL EQVSKLAGVL VKHGIKKGDT VVIYMPMIPQ
   181  AMYTMLACAR IGAIHSLIFG GFASKELSSR IDHVKPKVVV TASFGIEPGR RVEYVPLVEE
   241  ALKIGQHKPD KILIYNRPNM EAVPLAPGRD LDWDEEMAKA QSHDCVPVLS EHPLYILYTS
   301  GTTGLPKGVI RPTGGYAVML HWSMSSIYGL QPGEVWWAAS DLGWVVGHSY ICYGPLLHGN
   361  TTVLYEGKPV GTPDAGAYFR VLAEHGVAAL FTAPTAIRAI RQQDPGAALG KQYSLTRFKT
   421  LFVAGERCDV ETLEWSKNVF RVPVLDHWWQ TETGSPITAS CVGLGNSKTP PPGQAGKSVP
   481  GYNVMILDDN MQKLKARCLG NIVVKLPLPP GAFSGLWKNQ EAFKHLYFEK FPGYYDTMDA
   541  GYMDEEGYLY VMSRVDDVIN VAGHRISAGA IEESILSHGT VADCAVVGKE DPLKGHVPLA
   601  LCVLRKDINA TEEQVLEEIV KHVRQNIGPV AAFRNAVFVK QLPKTRSGKI PRSALSAIVN
   661  GKPYKITSTI EDPSIFGHVE EMLKQA

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ACSS3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.26

Highest tissue expression

37 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.95

gnomAD pLI

0

gnomAD missense Z

0.15

DepMap mean gene effect

0.07

DepMap dependency class

none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• ketone body biosynthetic process

Molecular functions

• acetate-CoA ligase activity
• ATP binding
• medium-chain fatty acid-CoA ligase activity
• propionate-CoA ligase activity

Cellular components

• mitochondrial matrix
• mitochondrion

Protein domainsUniProt · Pfam · InterPro

• AMP-dependent synthetase/ligase domain
• AMP-binding, conserved site
• AMP-binding enzyme, C-terminal domain
• Acetyl-coenzyme A synthetase, N-terminal domain
• ANL, N-terminal domain
• AMP-binding enzyme domain superfamily
• AMP-binding enzyme
• AMP-binding enzyme C-terminal domain
• Acetyl-coenzyme A synthetase N-terminus

KeywordsUniProt

• Acetylation
• ATP-binding
• Ligase
• Lipid metabolism
• Mitochondrion
• Nucleotide-binding
• Transit peptide