Seroatlas · Human Serome Atlas

WNT5A

Protein Wnt-5a

Also known as: hWNT5A, WNT5A_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
P41221
Gene
WNT5A
Ensembl
ENSG00000114251
Chromosome
3
Canonical length
380 aa
Protein class
Disease related genes, Human disease related genes, Plasma proteins, Predicted secreted proteins
Subcellular location
Nucleoli fibrillar center,Microtubules,Mitotic spindle,Primary cilium
Secretome location
Secreted to extracellular matrix
Quaternary structure
Homooligomer

OverviewNCBI Gene

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene encodes a member of the WNT family that signals through both the canonical and non-canonical WNT pathways. This protein is a ligand for the seven transmembrane receptor frizzled-5 and the tyrosine kinase orphan receptor 2. This protein plays an essential role in regulating developmental pathways during embryogenesis. This protein may also play a role in oncogenesis. Mutations in this gene are the cause of autosomal dominant Robinow syndrome. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012]

Canonical amino-acid sequenceUniProt

380 residues, UniProt reviewed canonical sequence.

>P41221|WNT5A
     1  MKKSIGILSP GVALGMAGSA MSSKFFLVAL AIFFSFAQVV IEANSWWSLG MNNPVQMSEV
    61  YIIGAQPLCS QLAGLSQGQK KLCHLYQDHM QYIGEGAKTG IKECQYQFRH RRWNCSTVDN
   121  TSVFGRVMQI GSRETAFTYA VSAAGVVNAM SRACREGELS TCGCSRAARP KDLPRDWLWG
   181  GCGDNIDYGY RFAKEFVDAR ERERIHAKGS YESARILMNL HNNEAGRRTV YNLADVACKC
   241  HGVSGSCSLK TCWLQLADFR KVGDALKEKY DSAAAMRLNS RGKLVQVNSR FNSPTTQDLV
   301  YIDPSPDYCV RNESTGSLGT QGRLCNKTSE GMDGCELMCC GRGYDQFKTV QTERCHCKFH
   361  WCCYVKCKKC TEIVDQFVCK

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against WNT5A can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Secreted
Secreted
Yes
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.33
Highest tissue expression
32 nTPM

Expression across tissuesHPA

Tissue

  • salivary gland: 32 nTPM
  • endometrium: 26 nTPM
  • ovary: 19 nTPM
  • urinary bladder: 18 nTPM
  • esophagus: 16 nTPM
  • vagina: 12 nTPM

Single-cell type

  • prostatic club cells: 206 nCPM
  • prostatic hillock cells: 141 nCPM
  • papillary tip epithelial cells: 118 nCPM
  • endometrial stromal cells: 114 nCPM
  • pituitary stem cells: 98 nCPM
  • esophageal apical cells: 91 nCPM

Immune cell

  • basophil: 0 nTPM
  • classical monocyte: 0 nTPM
  • eosinophil: 0 nTPM
  • gdT-cell: 0 nTPM
  • intermediate monocyte: 0 nTPM
  • MAIT T-cell: 0 nTPM

Brain region

  • white matter: 14 nTPM
  • midbrain: 12 nTPM
  • medulla oblongata: 11 nTPM
  • spinal cord: 11 nTPM
  • hypothalamus: 9.7 nTPM
  • pons: 9 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about WNT5A.

Disease | AllUniProt

Conditions WNT5A is implicated in, by any mechanism.

  • Robinow syndrome, autosomal dominant 1 (DRS1) MIM:180700

Disease | GeneticClinVar

12 pathogenic / likely-pathogenic of 305 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.27
gnomAD pLI
0.99
gnomAD missense Z
1.98
DepMap mean gene effect
0.03
DepMap dependency class
none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of WNT5A in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads WNT5A as an antibody target. Whether an autoantibody or antibody against WNT5A could matter depends on whether native WNT5A is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

WNT5A is annotated as secreted, so native WNT5A circulates and is directly accessible to antibodies. Secreted and cell-surface proteins are the autoantibody targets most likely to act like drugs, blocking or depleting the native protein.

Annotation status

The present source text does not explicitly label WNT5A as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/WNT5A. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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