Seroatlas · Human Serome Atlas

WFIKKN1

WAP, Kazal, immunoglobulin, Kunitz and NTR domain-containing protein 1

Also known as: C16orf12, RJD2, WFDC20A, WFIKKN, WFKN1_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q96NZ8
Gene
WFIKKN1
Ensembl
ENSG00000127578
Chromosome
16
Canonical length
548 aa
Protein class
Predicted secreted proteins
Secretome location
Secreted in other tissues

OverviewNCBI Gene

This gene encodes a secreted multidomain protein consisting of a signal peptide, a WAP domain, a follistatin domain, an immunoglobulin domain, two tandem Kunitz domains, and an NTR domain. These domains have been implicated frequently in inhibition of various types of proteases, suggesting that the encoded protein may be a multivalent protease inhibitor and may control the action of multiple types of serine proteases as well as metalloproteinases. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

548 residues, UniProt reviewed canonical sequence.

>Q96NZ8|WFIKKN1
     1  MPALRPLLPL LLLLRLTSGA GLLPGLGSHP GVCPNQLSPN LWVDAQSTCE RECSRDQDCA
    61  AAEKCCINVC GLHSCVAARF PGSPAAPTTA ASCEGFVCPQ QGSDCDIWDG QPVCRCRDRC
   121  EKEPSFTCAS DGLTYYNRCY MDAEACLRGL HLHIVPCKHV LSWPPSSPGP PETTARPTPG
   181  AAPVPPALYS SPSPQAVQVG GTASLHCDVS GRPPPAVTWE KQSHQRENLI MRPDQMYGNV
   241  VVTSIGQLVL YNARPEDAGL YTCTARNAAG LLRADFPLSV VQREPARDAA PSIPAPAECL
   301  PDVQACTGPT SPHLVLWHYD PQRGGCMTFP ARGCDGAARG FETYEACQQA CARGPGDACV
   361  LPAVQGPCRG WEPRWAYSPL LQQCHPFVYG GCEGNGNNFH SRESCEDACP VPRTPPCRAC
   421  RLRSKLALSL CRSDFAIVGR LTEVLEEPEA AGGIARVALE DVLKDDKMGL KFLGTKYLEV
   481  TLSGMDWACP CPNMTAGDGP LVIMGEVRDG VAVLDAGSYV RAASEKRVKK ILELLEKQAC
   541  ELLNRFQD

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against WFIKKN1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Secreted
Secreted
Yes
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.37
Highest tissue expression
5 nTPM

Expression across tissuesHPA

Tissue

  • cerebellum: 5 nTPM
  • kidney: 2.1 nTPM
  • basal ganglia: 1.8 nTPM
  • cerebral cortex: 1.8 nTPM
  • amygdala: 1.4 nTPM
  • pituitary gland: 1.4 nTPM

Single-cell type

  • thymic myoid cells: 14 nCPM
  • medullary thymic epithelial cells: 9.6 nCPM
  • retinal horizontal cells: 8 nCPM
  • foveolar cells: 6 nCPM
  • respiratory ionocytes: 5.1 nCPM
  • gonadotrophs: 4.2 nCPM

Immune cell

  • basophil: 0 nTPM
  • classical monocyte: 0 nTPM
  • eosinophil: 0 nTPM
  • gdT-cell: 0 nTPM
  • intermediate monocyte: 0 nTPM
  • MAIT T-cell: 0 nTPM

Brain region

  • cerebellum: 4.3 nTPM
  • cerebral cortex: 2.7 nTPM
  • white matter: 2.1 nTPM
  • basal ganglia: 1.8 nTPM
  • amygdala: 1.6 nTPM
  • hippocampal formation: 1.6 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
1.14
gnomAD pLI
0
gnomAD missense Z
-0.45
DepMap mean gene effect
0.02
DepMap dependency class
none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads WFIKKN1 as an antibody target. Whether an autoantibody or antibody against WFIKKN1 could matter depends on whether native WFIKKN1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

WFIKKN1 is annotated as secreted, so native WFIKKN1 circulates and is directly accessible to antibodies. Secreted and cell-surface proteins are the autoantibody targets most likely to act like drugs, blocking or depleting the native protein.

Annotation status

The present source text does not explicitly label WFIKKN1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/WFIKKN1. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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