Seroatlas · Human Serome Atlas

WFDC8

WAP four-disulfide core domain protein 8

Also known as: C20orf170, dJ461P17.1, WAP8, WFDC8_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q8IUA0
Gene
WFDC8
Ensembl
ENSG00000158901
Chromosome
20
Canonical length
241 aa
Protein class
Predicted secreted proteins
Secretome location
Secreted in male reproductive system

OverviewNCBI Gene

This gene encodes a member of the WAP-type four-disulfide core (WFDC) domain family. The WFDC domain, or WAP signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor. The encoded protein contains a Kunitz-inhibitor domain, in addition to three WFDC domains. Most WFDC genes are localized to chromosome 20q12-q13 in two clusters: centromeric and telomeric. This gene belongs to the telomeric cluster. Two alternatively spliced transcript variants have been found for this gene, and they encode the same protein. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

241 residues, UniProt reviewed canonical sequence.

>Q8IUA0|WFDC8
     1  MWTVRTEGGH FPLHSPTFSW RNVAFLLLLS LALEWTSAML TKKIKHKPGL CPKERLTCTT
    61  ELPDSCNTDF DCKEYQKCCF FACQKKCMDP FQEPCMLPVR HGNCNHEAQR WHFDFKNYRC
   121  TPFKYRGCEG NANNFLNEDA CRTACMLIVK DGQCPLFPFT ERKECPPSCH SDIDCPQTDK
   181  CCESRCGFVC ARAWTVKKGF CPRKPLLCTK IDKPKCLQDE ECPLVEKCCS HCGLKCMDPR
   241  R

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against WFDC8 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Secreted
Secreted
Yes
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.38
Highest tissue expression
387 nTPM

Expression across tissuesHPA

Tissue

  • epididymis: 387 nTPM
  • seminal vesicle: 0.7 nTPM
  • thyroid gland: 0.5 nTPM
  • testis: 0.2 nTPM
  • bone marrow: 0.1 nTPM
  • fallopian tube: 0.1 nTPM

Single-cell type

  • epididymal principal cells: 529 nCPM
  • endometrial luminal cells: 19 nCPM
  • epididymal clear cells: 8.2 nCPM
  • epididymal basal cells: 8 nCPM
  • salivary basal cells: 5.8 nCPM
  • smooth muscle cells: 5.2 nCPM

Immune cell

  • classical monocyte: 0.1 nTPM
  • total PBMC: 0.1 nTPM
  • basophil: 0 nTPM
  • eosinophil: 0 nTPM
  • gdT-cell: 0 nTPM
  • intermediate monocyte: 0 nTPM

Brain region

  • cerebral cortex: 1.8 nTPM
  • white matter: 1.7 nTPM
  • medulla oblongata: 1.5 nTPM
  • basal ganglia: 1.4 nTPM
  • cerebellum: 1.4 nTPM
  • hippocampal formation: 1.4 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
1.42
gnomAD pLI
0
gnomAD missense Z
0.68
DepMap mean gene effect
0.02
DepMap dependency class
none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads WFDC8 as an antibody target. Whether an autoantibody or antibody against WFDC8 could matter depends on whether native WFDC8 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

WFDC8 is annotated as secreted, so native WFDC8 circulates and is directly accessible to antibodies. Secreted and cell-surface proteins are the autoantibody targets most likely to act like drugs, blocking or depleting the native protein.

Annotation status

The present source text does not explicitly label WFDC8 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/WFDC8. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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